Phage-based capacitive biosensor for Salmonella detection

Talanta
Saroh NiyomdechaPanote Thavarungkul

Abstract

This article reports the detection of Salmonella spp. based on M13 bacteriophage in a capacitive flow injection system. Salmonella-specific M13 bacteriophage was immobilized on a polytyramine/gold surface using glutaraldehyde as a crosslinker. The M13 bacteriophage modified electrode can specifically bind to Salmonella spp. via the amino acid groups on the filamentous phage. An alkaline solution was used to break the binding between the sensing surface and the analyte to allow renewable use up to 40 times. This capacitive system provided good reproducibility with a relative standard deviation (RSD) of 1.1%. A 75 µL min-1 flow rate and a 300 µL sample volume provided a wide linear range, from 2.0 × 102 to 1.0 × 107 cfu mL-1, with a detection limit of 200 cfu mL-1. Bacteria concentration can be analyzed within 40 min after the sample injection. When applied to test real samples (raw chicken meat) it provided good recoveries (100-111%). An enrichment process was also explored to increase the bacteria concentration, enabling a quantitative detection of Salmonella spp. This biosensor opens a new opportunity for the detection of pathogenic bacteria using bacteriophage.

Citations

Oct 17, 2019·Nanomaterials·Jong-Sik MoonJin-Woo Oh
Jun 21, 2019·Viruses·Edel StoneOlivia McAuliffe
Dec 11, 2019·Micromachines·Jingting XuYi-Kuen Lee
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Yafang ShenYanbin Li
Jan 12, 2021·Biosensors & Bioelectronics·Wajid HussainShenqi Wang
May 1, 2021·Pharmaceuticals·Daniel Rosner, Jason Clark
May 27, 2021·ACS Biomaterials Science & Engineering·Larry O'ConnellYoann Roupioz

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