Phage display technique identifies the interaction of severe acute respiratory syndrome coronavirus open reading frame 6 protein with nuclear pore complex interacting protein NPIPB3 in modulating Type I interferon antagonism

Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi
Su-Hua HuangCheng-Wen Lin

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) proteins including ORF6 inhibit Type I interferon (IFN) signaling. This study identified SARS-CoV ORF6-interacting proteins using the phage displayed human lung cDNA libraries, and examined the association of ORF6-host factor interaction with Type I IFN antagonism. After the fifth round of biopanning with Escherichia coli-synthesized ORF6-His tagged protein, the relative binding affinity of phage clones to ORF6 was determined using direct enzyme-linked immunosorbent assay. The highest affinity clone to ORF6 displayed the C-terminal domain of NPIPB3 (nuclear pore complex interacting protein family, member B3; also named as phosphatidylinositol-3-kinase-related kinase SMG-1 isoform 1 homolog). The coimmunoprecipitation assay demonstrated the direct binding of ORF6 to the C-terminal domain of NPIPB3 in vitro. Confocal imaging revealed a close colocalization of SARS-CoV ORF6 protein with NPIPB3 in human promonocytes. The dual luciferase reporter assay showed that the C-terminal domain of NPIPB3 attenuated the antagonistic activity of SARS-CoV ORF6 on IFN-β-induced ISRE (IFN stimulated response element)-responsive firefly luciferase activity. In addition, confocal imaging and ...Continue Reading

References

Aug 25, 2000·Human Molecular Genetics·D J ElliottH J Cooke
May 1, 2002·The Journal of Biological Chemistry·Liliane A DickinsonJoel M Gottesfeld
Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
May 6, 2003·Science·Marco A MarraRachel L Roper
Dec 24, 2004·Nature·Joel MartinLen A Pennacchio
Feb 15, 2005·Antiviral Research·Yee-Joo TanWanjin Hong
Nov 30, 2007·Virus Research·Krishna NarayananShinji Makino
Aug 19, 2008·Biochimica Et Biophysica Acta·Zhongde YeYong Xie
Apr 3, 2012·Journal of Virological Methods·Ying-Ju LinCheng-Wen Lin
Jul 6, 2014·Antiviral Research·Ding Xiang LiuRolf Hilgenfeld
Dec 3, 2014·Current Opinion in Structural Biology·Domagoj Baretić, Roger L Williams

❮ Previous
Next ❯

Citations

Sep 19, 2019·Briefings in Functional Genomics·Cemalettin Bekpen, Diethard Tautz
Jul 24, 2020·Journal of Virology·Abigail VanderheidenMehul S Suthar
Apr 4, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Garret N GomezAnita Nag
Jun 18, 2020·PeerJ·Müşerref Duygu Saçar Demirci, Aysun Adan
Aug 20, 2020·Journal of Translational Medicine·Laure Perrin-CoconPierre-Olivier Vidalain
Mar 10, 2021·Current Microbiology·Taruna AnandBhupendra N Tripathi
May 31, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yusuf Oloruntoyin AyipoMohd Nizam Mordi
Jun 9, 2020·Journal of Medicinal Chemistry·Carmen GilAna Martinez

❮ Previous
Next ❯

Datasets Mentioned

BETA
Q92617

Methods Mentioned

BETA
phage display
coimmunoprecipitation
nuclear translocation
enzyme-linked immunosorbent assay
Assay
PCR
electrophoresis
ELISA
confocal microscopy

Software Mentioned

BLAST
SPSS

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.