Phage-Displayed Peptides Selected to Bind Envelope Glycoprotein Show Antiviral Activity against Dengue Virus Serotype 2

Advances in Virology
Carolina de la GuardiaRicardo Lleonart

Abstract

Dengue virus is a growing public health threat that affects hundreds of million peoples every year and leave huge economic and social damage. The virus is transmitted by mosquitoes and the incidence of the disease is increasing, among other causes, due to the geographical expansion of the vector's range and the lack of effectiveness in public health interventions in most prevalent countries. So far, no highly effective vaccine or antiviral has been developed for this virus. Here we employed phage display technology to identify peptides able to block the DENV2. A random peptide library presented in M13 phages was screened with recombinant dengue envelope and its fragment domain III. After four rounds of panning, several binding peptides were identified, synthesized, and tested against the virus. Three peptides were able to block the infectivity of the virus while not being toxic to the target cells. Blind docking simulations were done to investigate the possible mode of binding, showing that all peptides appear to bind domain III of the protein and may be mostly stabilized by hydrophobic interactions. These results are relevant to the development of novel therapeutics against this important virus.

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Citations

May 20, 2020·International Journal of Peptide Research and Therapeutics·Garima Agarwal, Reema Gabrani
Dec 6, 2018·Current Medicinal Chemistry·Adib Afandi AbdullahChoon Han Heh
Jan 1, 2021·Journal of Biomolecular Structure & Dynamics·Aathe Cangaree ArumugamAbdullah Al Hadi Ahmad Fuaad
Jul 3, 2021·Viruses·Esen SokulluBenoit Coulombe

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Methods Mentioned

BETA
FCS
affinity purification
Assay
phage-ELISA
phage display
ELISA
amidation

Software Mentioned

TASSER
GeneOptimizer
LigPlot +
JalView
GraphPad Prism
dock
CABS

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