Phage-encoded Serine Integrases and Other Large Serine Recombinases

Microbiology Spectrum
Margaret C M Smith

Abstract

The large serine recombinases (LSRs) are a family of enzymes, encoded in temperate phage genomes or on mobile elements, that precisely cut and recombine DNA in a highly controllable and predictable way. In phage integration, the LSRs act at specific sites, the attP site in the phage and the attB site in the host chromosome, where cleavage and strand exchange leads to the integrated prophage flanked by the recombinant sites attL and attR. The prophage can excise by recombination between attL and attR but this requires a phage-encoded accessory protein, the recombination directionality factor (RDF). Although the LSRs can bind specifically to all the recombination sites, only specific integrase-bound sites can pair in a synaptic complex prior to strand exchange. Recent structural information has led to a breakthrough in our understanding of the mechanism of the LSRs, notably how the LSRs bind to their substrates and how LSRs display this site-selectivity. We also understand that the RDFs exercise control over the LSRs by protein-protein interactions. Other recent work with the LSRs have contributed to our understanding of how all serine recombinases undergo strand exchange subunit rotation, facilitated by surfaces that resemble a ...Continue Reading

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D L Popham, P Stragier
Dec 20, 1991·Journal of Molecular Biology·S Kuhstoss, R N Rao
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·C D CarrascoJ W Golden
Jan 1, 1994·Genes & Development·C D CarrascoJ W Golden
Mar 1, 1997·Molecular Microbiology·K S RamaswamyJ W Golden
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·H M Thorpe, M C Smith
May 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·A C GrothM P Calos
Apr 26, 2002·Molecular Microbiology·Margaret C M Smith, Helena M Thorpe
Oct 2, 2002·Molecular Microbiology·Lori A Bibb, Graham F Hatfull
Feb 6, 2003·Cellular and Molecular Life Sciences : CMLS·P MullanyH Wang
Feb 6, 2003·Cellular and Molecular Life Sciences : CMLS·V AdamsJ I Rood
Aug 19, 2003·Journal of Bacteriology·Matthew A GregoryMargaret C M Smith
Nov 18, 2003·Molecular Microbiology·Amy I KimGraham F Hatfull
Dec 23, 2003·Journal of Molecular Biology·Amy C Groth, Michele P Calos
Mar 11, 2004·Molecular Microbiology·Matthew C A SmithMargaret C M Smith
May 14, 2004·Nucleic Acids Research·Matthew C A SmithMargaret C M Smith
Mar 9, 2005·Molecular Microbiology·Lori A BibbGraham F Hatfull
May 14, 2005·Journal of Molecular Biology·Pallavi GhoshGraham F Hatfull
Jan 20, 2006·FEMS Immunology and Medical Microbiology·Anne-Merethe Hanssen, Johanna U Ericson Sollid
Apr 20, 2006·Journal of Bacteriology·Derrick E FoutsSteven R Gill
May 25, 2006·PLoS Biology·Pallavi GhoshGraham F Hatfull
Jun 8, 2006·Annual Review of Biochemistry·Nigel D F GrindleyPhoebe A Rice

❮ Previous
Next ❯

Citations

Nov 1, 2016·Genome Announcements·Ivan Erill, Steven M Caruso
Jul 9, 2016·Nucleic Acids Research·Alexandra PokhilkoSean D Colloms
Jan 10, 2018·Nucleic Acids Research·Caitlin S TrejoPhoebe A Rice
Dec 23, 2016·Applied and Environmental Microbiology·Paul C M FoggMargaret C M Smith
Jan 13, 2017·Journal of the Royal Society, Interface·Alexandra PokhilkoOliver Ebenhöh
Jun 8, 2018·Journal of the Royal Society, Interface·Alexandra PokhilkoSean D Colloms
May 27, 2017·Nucleic Acids Research·Kushol GuptaGregory D Van Duyne

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Related Papers

Critical Reviews in Biochemistry and Molecular Biology
Gregory D Van Duyne, Karen Rutherford
Molecular Microbiology
W Marshall Stark
© 2021 Meta ULC. All rights reserved