Phage nanofibers in nanomedicine: Biopanning for early diagnosis, targeted therapy, and proteomics analysis.
Abstract
Display of a peptide or protein of interest on the filamentous phage (also known as bacteriophage), a biological nanofiber, has opened a new route for disease diagnosis and therapy as well as proteomics. Earlier phage display was widely used in protein-protein or antigen-antibody studies. In recent years, its application in nanomedicine is becoming increasingly popular and encouraging. We aim to review the current status in this research direction. For better understanding, we start with a brief introduction of basic biology and structure of the filamentous phage. We present the principle of phage display and library construction method on the basis of the filamentous phage. We summarize the use of the phage displayed peptide library for selecting peptides with high affinity against cells or tissues. We then review the recent applications of the selected cell or tissue targeting peptides in developing new targeting probes and therapeutics to advance the early diagnosis and targeted therapy of different diseases in nanomedicine. We also discuss the integration of antibody phage display and modern proteomics in discovering new biomarkers or target proteins for disease diagnosis and therapy. Finally, we propose an outlook for furt...Continue Reading
References
Identification of a peptide blocking vascular endothelial growth factor (VEGF)-mediated angiogenesis
Recognition of cell-specific binding of phage display derived peptides using an acoustic wave sensor
Identification of novel carrier peptides for the specific delivery of therapeutics into cancer cells
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