Phage types, ribotypes and tetracycline resistance genes of Salmonella enterica subsp. enterica serovar Typhimurium strains isolated from different origins in Italy

Veterinary Microbiology
Frédérique PasqualiGerardo Manfreda

Abstract

The tetracycline resistance (tet) gene patterns of 52 tetracycline resistant Salmonella enterica subsp. enterica (S.) serovar Typhimurium isolates collected from animals, food of animal origin, and humans in Italy, were investigated to evaluate whether the tet gene patterns could be used for strain differentiation in addition to phage typing and ribotyping. The detection of tet genes was performed by specific PCR assays. Ribotyping was performed automatically using PvuII as restriction enzyme. Ten different ribotyping patterns were detected. All isolates were positive for at least one of the tet genes studied and six different tet gene patterns were observed. Ribotyping and tet gene patterns showed discriminatory indices of 0.741 and 0.812, respectively. Multiple tet genes were commonly found among tetracycline resistant S. typhimurium isolates from various sources. The resulting tet gene patterns allowed further discrimination of strains which were otherwise indistinguishable by their phage type, ribotype and origin. Thus, the analysis of tet gene patterns might represent an additional tool for the differentiation of S. typhimurium isolates.

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Citations

Jul 10, 2007·International Journal of Environmental Health Research·Jonas D AlbarnazCélia R M Barardi
May 24, 2006·Microbes and Infection·Geovana Brenner MichaelStefan Schwarz
Apr 27, 2013·Comparative Immunology, Microbiology and Infectious Diseases·Se-Yeoun ChaHyung-Kwan Jang
Jul 6, 2017·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Pierangeli G VitalWindell L Rivera
Apr 20, 2005·FEMS Microbiology Letters·Marilyn C Roberts
May 26, 2005·The Journal of Antimicrobial Chemotherapy·Keith Poole

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