Phagocyte-induced antigen-specific activation of unprimed CD8+ T cells in vitro

European Journal of Immunology
M L De BruijnP A Peterson

Abstract

The strict segregation of the major histocompatibility complex (MHC) class I and class II loading pathways has been challenged by recent reports indicating that MHC class I molecules can acquire antigen in the phagocytic pathway. We now show that this alternative peptide loading pathway can be used efficiently to generate macrophages able to activate unprimed antigen-specific cytotoxic T cells in vitro. Short peptides (8-11 residues), administered in the phagocytic pathway at nanomolar concentrations, were found to be effective in specifically activating naïve cytotoxic T lymphocytes (CTL) in vitro, but longer peptides or whole protein antigen were not. Whole protein antigen coated on beads did, however, render macrophages susceptible to lysis by an antigen-specific CTL clone. This indicates that proteolysis in the phagocytic pathway has limited capability for class I-restricted presentation. We propose a model for class I loading in the phagocytic pathway consisting of direct trafficking of nascent MHC class I from the trans-Golgi network to the phagosome, prior to appearance at the cell surface, and the use of the narrow cavity between bead and phagosomal membrane as a peptide exchange/loading compartment. Targeting immunogen...Continue Reading

References

Mar 1, 1992·The Journal of Experimental Medicine·N A Hosken, M J Bevan
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J D SmithT H Hansen
Nov 1, 1992·European Journal of Immunology·M L De BruijnC J Melief
Sep 1, 1992·European Journal of Immunology·M L De BruijnC J Melief
Sep 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·L D FaloK L Rock
Jul 27, 1990·Cell·J W Yewdell, J R Bennink
Apr 25, 1991·Nature·T N SchumacherH L Ploegh
Apr 1, 1991·Immunology Today·T J Braciale, V L Braciale
Apr 11, 1991·International Immunology·Y Liu, C A Janeway
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·V Ortiz-Navarrete, G J Hämmerling
Jul 27, 1990·Cell·A TownsendA Tse
Aug 2, 1990·Nature·H G LjunggrenK Kärre
Jun 1, 1988·The Journal of Experimental Medicine·F R CarboneM J Bevan
Feb 1, 1989·Current Opinion in Immunology·S H Kaufmann
Jan 1, 1989·Current Opinion in Immunology·A Mantovani
Apr 1, 1989·The Journal of Experimental Medicine·S E MacatoniaB A Askonas
Aug 1, 1987·The Journal of Cell Biology·K SandvigB van Deurs
Jul 1, 1987·The Journal of Experimental Medicine·K InabaR M Steinman
Jan 1, 1980·Immunological Reviews·R M Steinman, M C Nussenzweig
Jan 1, 1984·Annual Review of Immunology·E R Unanue
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M C NussenzweigB Gutchinov
Jan 28, 1994·Cell·C A Janeway, K Bottomly
Jun 1, 1994·Current Opinion in Immunology·M Croft
Aug 1, 1994·Current Opinion in Immunology·S H Kaufmann
Jun 1, 1993·Current Opinion in Immunology·S C Knight, A J Stagg
Jun 1, 1993·The Journal of Experimental Medicine·L G EissenbergP H Schlesinger
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M Kovacsovics-BankowskiK L Rock

❮ Previous
Next ❯

Citations

Mar 1, 1996·Journal of Clinical Immunology·C V Harding
Feb 27, 2001·Journal of Immunological Methods·P K WallaceM W Fanger
Aug 30, 2002·Molecular Immunology·Monique Grommé, Jacques Neefjes
Jun 1, 1996·Current Opinion in Immunology·A Lanzavecchia
Mar 15, 2001·Traffic·M J KleijmeerH J Geuze
Oct 1, 1996·Immunity·M JondalJ Reimann
Jan 19, 2005·World Journal of Gastroenterology : WJG·Bao-Gang PengJie-Fu Huang
Jun 1, 1996·Immunological Reviews·L L Lenz, M J Bevan
Jul 6, 2004·Immunological Reviews·Jens A LeifertJ Lindsay Whitton
Sep 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·I PopovR D Inman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.