Phagocytic activity and oxidative burst of granulocytes in persons with myeloperoxidase deficiency

European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
C E GerberG Bruchelt

Abstract

In the present study, phagocytosis and the oxidative metabolism of neutrophil granulocytes from five clinically healthy persons with different degrees of myeloperoxidase deficiency were investigated and compared to those of normal persons. The identification of individuals with myeloperoxidase deficiency was performed with the Bayer/Technicon H3 blood cell counter, which differentiates the leukocytes by measuring the peroxidase activity. Neutrophils of three out of five investigated myeloperoxidase deficient persons showed extremely low peroxidase indices (-53 and lower), but only the neutrophils of one person totally lacked myeloperoxidase. This was demonstrated by comparing myeloperoxidase mass concentration measured with an enzyme immunoassay, lack of HOCl production, and was further confirmed by measuring luminol- and lucigenin-enhanced chemiluminescence. Characteristically, myeloperoxidase deficient granulocytes showed a strikingly decreased luminol-enhanced chemiluminescence while the lucigenin-enhanced chemiluminescence was significantly increased compared to normal granulocytes. Although there is a DNA sequence homology of about 70%, the activity of peroxidase in eosinophils was not affected in any myeloperoxidase defic...Continue Reading

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Citations

Mar 24, 2009·Nature Medicine·Shimon GrossDavid Piwnica-Worms
Sep 30, 2014·Journal of Immunological Methods·Jörg FlemmigJürgen Arnhold
Nov 25, 2003·Kidney International·Ernst MalleHermann-Josef Grone
Jan 15, 2009·Phytotherapy Research : PTR·Asif AhmadM Ahmed Mesaik
May 14, 2016·International Immunopharmacology·Almas JabeenShaheen Faizi
Feb 24, 2015·The Journal of Pharmacology and Experimental Therapeutics·Wei ZhengLeonard Buckbinder
Aug 14, 2008·Phytotherapy Research : PTR·M Ahmed MesaikS Mohiuddin

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