PMID: 6399274Jan 1, 1984Paper

Phagocytic and intracellular killing activity of rat macrophages: critical analysis of 2 different evaluation methods

Giornale Di Batteriologia, Virologia Ed Immunologia
D IannelloM Carbone

Abstract

Two different evaluation methods of the "in vitro" bacterial killing activity of macrophages were compared; the former based on the determination of the number of viable microorganisms in the supernatant of macrophage cultures by a microbiological plate method; the latter based on the evidentiation of intracellular killing by differential staining of living and killed microorganisms with acridine orange. Phagocytic and microbicidal activities of peritoneal cells were investigated by the two methods in control rats and in tumor-bearing rats. Qualitative and quantitative differences in the kinetics of phagocytosis and microbial killing were evidentiated in macrophages from tumor-bearing rats. Furthermore, both methods proved to be suitable and reproducible.

Related Concepts

Related Feeds

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.