Phagocytosis and intracellular killing of Candida albicans by murine polymorphonuclear neutrophils

Methods in Molecular Biology
Alieke G VonkBart Jan Kullberg

Abstract

Polymorphonuclear neutrophils (PMNs) are important phagocytes in the control of Candida infections. The phagocytic contribution of PMNs to host defence can by assessed by various methods, such as microbiological assays. However, assessment and definition of intracellular killing capacity can be a source of considerable confusion. A comparison of the growth of Candida in the presence of PMN with the growth of Candida in phagocyte-free suspensions may lead to an overestimation of killing capacity because PMNs can use both intracellular and extracellular killing mechanisms. Here, we describe the use of an adherent monolayer of exudate peritoneal PMNs that is used to differentiate between the process of phagocytosis and intracellular killing.

Citations

Jan 24, 2015·The Journal of Infectious Diseases·Joan Hui Juan LimLouis Yi Ann Chai
Apr 18, 2018·EMBO Molecular Medicine·Dayanira Alsina-BeauchampAna Cuenda
Aug 19, 2014·Nature Genetics·Gerald WirnsbergerJosef M Penninger
Mar 5, 2021·Bio-protocol·Sheng-Yang Wu, Betty A Wu-Hsieh
Aug 20, 2020··Betty Wu-HsiehSheng-Yang Wu

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