PMID: 9159191Apr 1, 1996Paper

Phagocytosis and intracellular killing of serum-opsonized Staphylococcus aureus by mouse fibroblasts expressing human Fcgamma receptor type IIa (CD32)

Frontiers in Bioscience : a Journal and Virtual Library
P H NibberingM F Geertsma

Abstract

Phagocytes bear more than one class of receptors for the Fc domain of IgG (FcgammaR). In addition the same ligand can interact with different classes of FcgammaR. This complexity makes it difficult to study the contribution of the various classes of FcgammaR to antimicrobial functions. To circumvent this difficulty, in the present study mouse 3T6 fibroblasts transfected with cDNA encoding for human FcgammaR type IIa (FcgammaRIIa-expressing cells) were used to determine the role of this receptor in phagocytosis and intracellular killing of serum-opsonized Staphylococcus aureus. Experiments using microbiological and fluorescent techniques to discriminate between cell-adherent and intracellular bacteria revealed that serum-opsonized bacteria are phagocytized by FcgammaRIIa-expressing cells, but not by parental fibroblasts. Non-opsonized bacteria were poorly internalized by FcgammaRIIa-expressing as well as parental fibroblasts. Furthermore, incubation of FcgammaRIIa-expressing cells with opsonized bacteria at 4oC and incubation of FcgammaRIIa-expressing cells with cytochalasin E prior to addition of opsonized bacteria inhibited the phagocytosis of these bacteria almost completely. Phagocytosis of opsonized bacteria by FcgammaRIIa-...Continue Reading

Citations

Dec 7, 2013·European Journal of Immunology·José L Maravillas-MonteroLeopoldo Santos-Argumedo
Aug 3, 2017·Parasites & Vectors·Abel Trujillo-OcampoFidel de la Cruz Hernández-Hernández
Jan 24, 2007·The Journal of Biological Chemistry·Edmond DupontAlain Joliot
Sep 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marijo ParcinaIsabelle Bekeredjian-Ding

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