PMID: 6413587Sep 30, 1983Paper

Phagocytosis by polymorphonuclear leukocytes of Staphylococcus aureus and Pseudomonas aeruginosa adherent to plastic, agar, or glass

Journal of Immunological Methods
D A LeeP K Peterson

Abstract

Phagocytic cells may encounter bacteria in vivo that are stationary or adherent to a surface. In this study, recently developed in vitro techniques were adapted to evaluate the interaction of polymorphonuclear leukocytes (PMN) with adherent Staphylococcus aureus and Pseudomonas aeruginosa. By measuring the uptake of radiolabeled bacteria, we found that normal human PMN readily phagocytize these organisms when they are attached to plastic or when they are grown on the surface of nutrient agar. Bacteria adherent to glass elicited a chemiluminescent response, and such organisms were phagocytized and killed by PMN. Opsonization of S. aureus and P. aeruginosa was not required for surface phagocytosis, chemiluminescence, or killing. These new methods should allow evaluation of certain biological and clinical aspects of surface phagocytosis in host defense.

References

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Citations

Apr 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A PascualJ Verhoef
Mar 1, 1990·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D L GordonV M Avery
Dec 1, 1991·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·V M Avery, D L Gordon
Oct 1, 1985·Veterinary Immunology and Immunopathology·N Craven, M R Williams
Aug 1, 1992·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·E T JensenJ W Costerton
Jun 13, 2009·PLoS Pathogens·Daniel M WeinbergerMarc Lipsitch
Aug 1, 1987·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·T Wadström
Apr 16, 2003·Eye & Contact Lens·Emma B H HumeMark D P Willcox
Jan 1, 1989·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·B JansenG Pulverer
Jan 1, 1989·Microbiology and Immunology·K SekiS Masuda
Nov 1, 1984·Infection and Immunity·P K PetersonR C Johnson
Mar 1, 1986·Infection and Immunity·C G WardJ J Bullen
Jan 1, 1994·Clinical and Diagnostic Laboratory Immunology·L C LimR F Schell
Dec 1, 1986·Infection and Immunity·I MaxeK Rubin
Oct 1, 1986·Infection and Immunity·G M JohnsonP G Quie

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