Phagocytosis by rat liver: relationships between phagosomes and lysosomes

Biochemical and Biophysical Research Communications
R WattiauxS Wattiaux-De Coninck

Abstract

To study the transfer of phagocytosed components from phagosomes to lysosomes, we have investigated phagocytosis by rat liver of killed Staphylococcus aureus labelled with (125)I tyramine cellobiose. Lysosomes were identified by injecting the animals with Triton WR1339, a non ionic detergent that is endocytosed by the liver and accumulates in lysosomes, causing a marked decrease of their density; that allows these organelles to be well separated from other particles in a density gradient. Bacteria were quickly taken up by the liver; their uptake is followed by a slow degradation as ascertained by the increase of acid-soluble radioactivity in the homogenates with time. Triton WR1339 injection does not affect the uptake and the degradation of the particles. Differential centrifugation of homogenates shows that at any time after injection, most of the radioactivity is recovered in the mitochondrial fractions. Distributions of acid precipitable and acid soluble radioactivities amongst subcellular structures present in mitochondrial fractions were studied by isopycnic centrifugation in sucrose gradients, at increasing times after bacteria injection. Results show that: 1) acid-precipitable radioactivity is quasi-exclusively present i...Continue Reading

Citations

Jan 1, 2003·Biochemical and Biophysical Research Communications·Richard D BagshawJohn W Callahan
Jun 6, 2009·The Biochemical Journal·Oliver SchieweckTorben Lübke
Feb 23, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·T E TjelleT Berg
Dec 8, 2004·Molecular & Cellular Proteomics : MCP·Richard D BagshawJohn W Callahan
May 9, 2003·The Journal of Biological Chemistry·Stephen H PasternakDon J Mahuran
Jun 30, 2000·Biochemical Pharmacology·R KjekenT Berg

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