Phagocytosis-independent antimicrobial activity of mast cells by means of extracellular trap formation

Blood
Maren von Köckritz-BlickwedeEva Medina

Abstract

These days it has been increasingly recognized that mast cells (MCs) are critical components of host defense against pathogens. In this study, we have provided the first evidence that MCs can kill bacteria by entrapping them in extracellular structures similar to the extracellular traps described for neutrophils (NETs). We took advantage of the ability of MCs to kill the human pathogen Streptococcus pyogenes by a phagocytosis-independent mechanism in order to characterize the extracellular antimicrobial activity of MCs. Close contact of bacteria and MCs was required for full antimicrobial activity. Immunofluorescence and electron microscopy revealed that S pyogenes was entrapped by extracellular structures produced by MCs (MCETs), which are composed of DNA, histones, tryptase, and the antimicrobial peptide LL-37. Disruption of MCETs significantly reduced the antimicrobial effect of MCs, suggesting that intact extracellular webs are critical for effective inhibition of bacterial growth. Similar to NETs, production of MCETs was mediated by a reactive oxygen species (ROS)-dependent cell death mechanism accompanied by disruption of the nuclear envelope, which can be induced after stimulation of MCs with phorbol-12-myristate-13-acet...Continue Reading

References

Apr 1, 1978·Infection and Immunity·W M Shafer, J J Iandolo
Jan 1, 1988·Leukemia Research·J H ButterfieldG J Gleich
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A A IraniL B Schwartz
Jan 1, 1969·Journal of Ultrastructure Research·A R Spurr
Mar 15, 1997·The Journal of Clinical Investigation·J A Cairns, A F Walls
Sep 1, 1997·Infection and Immunity·S N Abraham, R Malaviya
Feb 3, 1998·The Korean Journal of Parasitology·J K RheeH C Kim
May 21, 1999·Archives of Biochemistry and Biophysics·L CregarW R Moore
Mar 17, 2000·Immunological Reviews·Y A Mekori, D D Metcalfe
Feb 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y OkayamaD D Metcalfe
Apr 9, 2001·Immunological Reviews·R Malaviya, S N Abraham
Feb 28, 2002·Trends in Immunology·Frédéric FégerMichel Arock
Feb 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna Di NardoRichard L Gallo
Sep 10, 2003·The International Journal of Biochemistry & Cell Biology·I PuxedduF Levi-Schaffer
Dec 1, 1958·The Journal of Experimental Medicine·J G HIRSCH
Jan 27, 2004·Veterinary Immunology and Immunopathology·Sandra JollyDaniel Desmecht
Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
Apr 23, 2004·Infection and Immunity·Oliver GoldmannEva Medina
Aug 11, 2004·The Journal of Biological Chemistry·Sibylla MartinelliShida Yousefi
Oct 2, 2004·Nature Reviews. Immunology·Jean S Marshall
Oct 6, 2005·Trends in Cell Biology·Paul Martin, S Joseph Leibovich
Nov 4, 2005·European Journal of Pharmacology·Michel CarvalhoFernando Cunha
Nov 28, 2006·Current Opinion in Immunology·Wojciech Dawicki, Jean S Marshall
Jan 4, 2007·The American Journal of Pathology·Balázs DömeJózsef Tímár
Jan 11, 2007·The Journal of Cell Biology·Tobias A FuchsArturo Zychlinsky
Jan 27, 2007·Biochemistry·Sylvie CampagnaAndré Aumelas
Mar 30, 2007·Science's STKE : Signal Transduction Knowledge Environment·Benjamin E Steinberg, Sergio Grinstein
May 26, 2007·The American Journal of Pathology·Frank SiebenhaarMarcus Maurer
Jul 17, 2007·Nature Reviews. Microbiology·Volker Brinkmann, Arturo Zychlinsky
Nov 6, 2007·Internal Medicine·Ryuichi KawamotoSyunpei Nakamura

❮ Previous
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Citations

Jul 31, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Oliver Soehnlein
Jun 5, 2013·Seminars in Immunopathology·Nora Branzk, Venizelos Papayannopoulos
Aug 5, 2009·Medical Microbiology and Immunology·Tim LögtersMartin Scholz
Mar 31, 2010·Marine Biotechnology·Ulysses W Sallum, Thomas T Chen
Jul 18, 2009·Cell Death and Differentiation·S YousefiH U Simon
Feb 5, 2011·Cell Death and Differentiation·Q RemijsenT Vanden Berghe
Oct 27, 2009·Nature Medicine·Jun XuCharles T Esmon
Feb 26, 2013·Nature Reviews. Immunology·Elzbieta Kolaczkowska, Paul Kubes
Aug 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tobias A FuchsDenisa D Wagner
Jun 22, 2010·The Journal of Biological Chemistry·Simran Jeet KaurSusanne R Talay
Dec 8, 2009·Biological Reviews of the Cambridge Philosophical Society·Enrico Crivellato, Domenico Ribatti
Oct 27, 2012·Science Translational Medicine·Allison M CampbellMark J Shlomchik
Oct 20, 2010·Infection and Immunity·Richard A JuneauW Edward Swords
Nov 23, 2011·Infection and Immunity·Delbert S Abi AbdallahEric Y Denkers
Sep 11, 2010·Journal of Innate Immunity·Evelien T M BerendsMaren von Köckritz-Blickwede
Jan 19, 2011·Journal of Innate Immunity·Sarah M McAlpineGunnar Nilsson
Oct 19, 2013·International Archives of Allergy and Immunology·Valdirene S MunizJosiane S Neves
Feb 18, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Prakash SahaAlberto Smith
Jun 2, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Tobias A FuchsDenisa D Wagner
May 26, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Simon F De MeyerDenisa D Wagner
Dec 17, 2009·Blood·Maren von Köckritz-BlickwedeVictor Nizet
May 12, 2012·PLoS Pathogens·Mirjam Urb, Donald C Sheppard
Feb 11, 2012·Critical Reviews in Immunology·Alasdair M Gilfillan, Michael A Beaven
Apr 25, 2012·Journal of Periodontology·Heliton G LimaVanessa S Lara
Mar 12, 2013·Autoimmune Diseases·Miguel Antonio Mesa, Gloria Vasquez
Feb 22, 2014·Apoptosis : an International Journal on Programmed Cell Death·Barbara KleinHubert H Kerschbaum
May 20, 2014·Nanoscale·Consol FarreraBengt Fadeel
Dec 3, 2013·Annals of the Rheumatic Diseases·Nishant Dwivedi, Marko Radic
May 23, 2014·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Neelakshi R Jog, Roberto Caricchio
Aug 15, 2014·Nature Communications·Calum T RobbValerie J Smith

❮ Previous
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