PMID: 1485331Jan 1, 1992

Phagocytosis of collagen fibrils by periosteal fibroblasts in long bone explants. Effect of concanavalin A

Tissue & Cell
V Everts, W Beertsen

Abstract

In an attempt to determine whether phagocytosis of collagen by fibroblasts involves binding of the fibril to the plasma membrane, the effect of the lectin concanavalin A (Con A) was studied in an in vitro model system. Metacarpal bone rudiments from 19-day-old mouse fetuses were incubated with varying concentrations of the lectin. Quantitative electron microscopic analysis indicated that Con A caused a dose-related increase in the amount of phagocytosed collagen fibrils in periosteal fibroblasts, suggesting either an enhanced uptake or a decreased intracellular breakdown of fibrils. Since a Con A-inducible increase was not seen in the combined presence of both the lectin and the proteinase inhibitor leupeptin, which is known to inhibit the intracellular digestion of phagocytosed fibrillar collagen, it is unlikely that Con A stimulated phagocytosis. Based on the finding that Con A interfered with the digestion of a synthetic substrate by the collagenolytic lysosomal enzyme cathepsin B it is suggested that the augmentation of intracellular fibrillar collagen under the influence of the lectin was due to a decreased intracellular digestion. Since Con A did not inhibit the uptake of collagen fibrils by the fibroblasts it is conclude...Continue Reading

References

Nov 1, 1979·Analytical Biochemistry·T E Cawston, A J Barrett
Mar 1, 1975·Experimental Cell Research·I Yahara, G M Edelman
Jul 15, 1991·Biochemical and Biophysical Research Communications·C J van Noorden, V Everts
Aug 1, 1989·Matrix : Collagen and Related Research·V EvertsW Beertsen
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M J Elices, M E Hemler
Sep 1, 1987·Collagen and Related Research·C C ChuaR L Ladda
Sep 1, 1985·Collagen and Related Research·V EvertsW Tigchelaar-Gutter
Oct 1, 1970·Experimental Cell Research·A E Powell, M A Leon
Oct 1, 1981·Journal of Ultrastructure Research·A H Melcher, J Chan

Citations

Aug 21, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Z GalewskaS Jaworski
Dec 26, 2001·The International Journal of Biochemistry & Cell Biology·Z GalewskaS Jaworski
Apr 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·E WattrangC Fossum
Sep 15, 2006·Molecular Biology of the Cell·Hyejin LeeJaro Sodek
Jul 14, 2010·Progress in Retinal and Eye Research·T L PonsioenL I Los
Nov 27, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Z GalewskaS Jaworski
Oct 23, 2001·Respiratory Research·P J Wolters, H A Chapman
Apr 2, 2003·The Journal of Cell Biology·Lars H EngelholmThomas H Bugge

Related Concepts

Cathepsin B-Like Proteinase
Plasma Membrane
Zyderm
Concanavalin A
Cytochalasin B
Isoferritin
Fibroblasts
Electron Microscopy
Periosteum
Phagocytosis

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