Phagocytosis of haemozoin (malarial pigment) enhances metalloproteinase-9 activity in human adherent monocytes: role of IL-1beta and 15-HETE.

Malaria Journal
Mauro PratoPaolo Arese

Abstract

It has been shown previously that human monocytes fed with haemozoin (HZ) or trophozoite-parasitized RBCs displayed increased matrix metalloproteinase-9 (MMP-9) enzyme activity and protein/mRNA expression and increased TNF production, and showed higher matrix invasion ability. The present study utilized the same experimental model to analyse the effect of phagocytosis of: HZ, delipidized HZ, beta-haematin (lipid-free synthetic HZ) and trophozoites on production of IL-1beta and MMP-9 activity and expression. The second aim was to find out which component of HZ was responsible for the effects. Native HZ freshly isolated from Plasmodium falciparum (Palo Alto strain, Mycoplasma-free), delipidized HZ, beta-haematin (lipid-free synthetic HZ), trophozoites and control meals such as opsonized non-parasitized RBCs and inert latex particles, were fed to human monocytes. The production of IL-1beta by differently fed monocytes, in presence or absence of specific MMP-9 inhibitor or anti-hIL-1beta antibodies, was quantified in supernatants by ELISA. Expression of IL-1beta was analysed by quantitative real-time RT-PCR. MMP-9 activity and protein expression were quantified by gelatin zymography and Western blotting. Monocytes fed with HZ or tr...Continue Reading

References

Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A F SlaterG B Henderson
Aug 1, 1990·Immunology Letters·D Kwiatkowski
Jun 15, 1989·The New England Journal of Medicine·G E GrauP H Lambert
May 1, 1995·Journal of Leukocyte Biology·A J GearingR Gilbert
May 1, 1994·Analytical Biochemistry·D E Kleiner, W G Stetler-Stevenson
Jun 21, 1996·The Journal of Biological Chemistry·A ItoY Mori
Jun 22, 1999·Molecular and Biochemical Parasitology·E SchwarzerP Arese
Jul 27, 1999·The Journal of Biological Chemistry·H Nagase, J F Woessner
Sep 4, 1999·Neuropathology and Applied Neurobiology·H BrownG Turner
Dec 3, 1999·Parasite Immunology·T ScorzaP De Baetselier
Aug 31, 2002·Glia·Gary A Rosenberg
Sep 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stefania MitolaFederico Bussolino
Sep 25, 2003·Cellular and Molecular Life Sciences : CMLS·F GimenezD Mazier
Sep 27, 2003·Journal of Neuroimmunology·Martin H DeiningerHermann J Schluesener
Oct 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maritza JaramilloMartin Olivier
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Oleksii A SkorokhodEvelin Schwarzer
Dec 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maritza JaramilloMartin Olivier
Jan 31, 2006·Expert Opinion on Therapeutic Targets·Meilang XueChristopher J Jackson
May 9, 2006·Cancer Metastasis Reviews·Elena I Deryugina, James P Quigley
Jul 26, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Philippe E Van den SteenAxel Pagenstecher
Dec 15, 2006·Journal of Cellular Physiology·Chunhong Yan, Douglas D Boyd

❮ Previous
Next ❯

Citations

Dec 9, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S Balachandar, A Katyal
Sep 3, 2013·Asian Pacific Journal of Tropical Biomedicine·Chuchard Punsawad
Aug 25, 2010·Infection and Immunity·Giuliana GiribaldiPaolo Arese
Dec 6, 2011·Pharmacology & Therapeutics·Nathalie GeurtsPhilippe E Van den Steen
Aug 3, 2011·Travel Medicine and Infectious Disease·Damian Bruce-Hickman
Apr 23, 2010·British Journal of Haematology·Enrico M NovelliDouglas J Perkins
Feb 11, 2015·Cell Biochemistry and Function·Alexandra C Schrimpe-RutledgeDavid W Wright
Mar 27, 2010·FEMS Microbiology Reviews·Hany M Elsheikha, Naveed Ahmed Khan
Oct 5, 2011·Asian Pacific Journal of Tropical Medicine·Mauro Prato
Jan 29, 2014·Fluids and Barriers of the CNS·Manuela Polimeni, Mauro Prato
Dec 31, 2009·PloS One·Abigail A LamikanraDavid J Roberts
Mar 8, 2013·Cell Biochemistry and Function·Amina KhadjaviMauro Prato
Oct 3, 2018·Parasitology·Sarah D'AlessandroNicoletta Basilico
Nov 24, 2020·Trends in Parasitology·Thao-Thy PhamPhilippe E Van den Steen
Jul 18, 2021·Malaria Journal·Caroline Lin Lin ChuaAndrew Teo

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
Assay
PCR
PCRs

Software Mentioned

Beacon Designer

Related Concepts

Related Feeds

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.

Blood Brain Barrier & Cytokines

Some cytokines are able to cross the blood brain barrier through transport systems and enter the cerebrospinal fluid and interstitial fluid spaces. Here is the latest research on cytokines crossing the blood brain barrier and how this can affect tissues within the CNS.