Phagocytosis of Histoplasma capsulatum yeasts and microconidia by human cultured macrophages and alveolar macrophages. Cellular cytoskeleton requirement for attachment and ingestion

The Journal of Clinical Investigation
S L NewmanW E Bullock

Abstract

Phagocytosis of Histoplasma capsulatum (Hc) yeasts and microconidia by human macrophages (M phi) was quantified by a fluorescence quenching technique. Phagocytosis of unopsonized Hc yeasts by monocyte-derived M phi and human alveolar M phi (AM) was rapid. After 60 min, 79% of cultured M phi and 59% of AM had ingested an average of 9.8 and 11 yeasts/M phi, respectively. In contrast, only 26% of monocytes ingested 4.5 yeasts/cell after 60 min. Phagocytosis of unopsonized microconidia by cultured M phi and by AM was equivalent. Monoclonal antibodies specific for the alpha-chains and beta-chain of the CD18 family of adhesion receptors inhibited the binding of Hc yeasts and microconidia to cultured M phi and AM. Thus, the M phi CD18 complex mediates recognition of both phases of this dimorphic fungus. Disruption of actin microfilaments with cytochalasin D inhibited both attachment and ingestion of yeasts by M phi. In contrast, nocodazole, which prevents polymerization of microtubules, did not inhibit binding or ingestion. Both drugs inhibited ingestion, but neither drug inhibited binding of C3b- and C3bi-coated sheep erythrocytes to complement receptors type one (CR1) or type three (CR3), respectively. Therefore, different signal tr...Continue Reading

References

Jan 1, 1978·The Journal of Experimental Medicine·J W van der MeerR van Furth
Aug 1, 1988·The Journal of Clinical Investigation·B L MyonesG D Ross
Dec 1, 1983·The Journal of Experimental Medicine·S D Wright, S C Silverstein
Jan 1, 1980·Journal of Immunological Methods·F Gmelig-Meyling, T A Waldmann
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H B FleitJ C Unkeless
Oct 1, 1982·The Journal of Experimental Medicine·S D Wright, S C Silverstein

❮ Previous
Next ❯

Citations

Aug 1, 1990·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G S Deepe, W E Bullock
Jun 24, 1991·Journal of Immunological Methods·J L SubizaE G De la Concha
Apr 26, 1995·Journal of Immunological Methods·D G Perry, W J Martin
Oct 22, 2003·Archives of Medical Research·Esperanza Duarte-EscalanteMaria Lucia Taylor
Aug 28, 1998·Research in Immunology·G S Deepe, R A Seder
Mar 19, 1999·Trends in Microbiology·S L Newman
Sep 6, 2000·Current Opinion in Microbiology·G S Deepe
Dec 13, 2006·Nature Reviews. Microbiology·Jim E CutlerBruce S Klein
Dec 20, 2005·The Journal of Infectious Diseases·George S Deepe, Reta S Gibbons
Jun 19, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·George S Deepe
Sep 3, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hong-Beom BaeEdward Abraham
Nov 14, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·G Stent, S M Crowe
Oct 15, 2003·Clinical Microbiology Reviews·Timothy Andrews, Kathleen E Sullivan
Jan 31, 2009·Infection and Immunity·Allan J GuimarãesJoshua D Nosanchuk
Jun 7, 2006·Annual Review of Microbiology·Chad A Rappleye, William E Goldman
Jan 28, 1999·American Journal of Respiratory Cell and Molecular Biology·A M LeVineT R Korfhagen
Aug 1, 1995·The Journal of Clinical Investigation·J P McMahonW J Martin
Jun 26, 2008·Journal of Leukocyte Biology·George S DeepeA George Smulian
Jan 10, 2012·Annual Review of Immunology·Marcel WüthrichBruce Klein
Oct 8, 2013·Current Opinion in Microbiology·Simon L Newman, A George Smulian
Aug 4, 2015·The FEBS Journal·Andrew L Garfoot, Chad A Rappleye
May 2, 2015·Virulence·Sharanjeet K ThindJoshua D Nosanchuk
Jun 25, 2008·Current Opinion in Microbiology·Eric D Holbrook, Chad A Rappleye
Mar 24, 2009·Microbiology and Immunology·Yuttana SrinoulprasertSansanee C Chaiyaroj
Jun 19, 2012·The Biochemical Journal·David CarlingMatthew J Sanders
Aug 13, 2015·Comparative Immunology, Microbiology and Infectious Diseases·Jorge Humberto SahazaMaria Lucia Taylor

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.