PMID: 7515698Mar 1, 1994Paper

Phagocytosis of intravenously administered particles by leukocytes adhered to the aortic endothelium of the rat

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
M GabaldónA Zúñiga

Abstract

Phagocytosis has been used to characterize on a functional basis leukocytes adhered to the aortic endothelium of the rat. After intravenous administration of particles, phagocytosis was observed microscopically in esterase-positive leukocytes adhered to the endothelium in whole mounts of aorta. Polybead blue and red, 0.5 and 1 microns particle size, were inadequate because they were insufficiently colored to be identified individually at 400 x. Fluoresbrite YG 0.25 and 0.50 micron at doses of 0.2 and 2 x 0.3 ml/100 g, respectively, produced endothelial lesions. The same occurred with Monastral blue B (MbB) at 0.3 ml/100 g, red iron at 2 x 16 mg/100 g and India ink at different concentrations depending on the supplier. At lower particle doses, lesions were not found. Deferoxamine mesylate 1.5 mg/100 g intravenous and allopurinol 5 mg/100 g intraperitoneal administered before the particles diminished the number and intensity of lesions. In none of the cases studied was the percentage of phagocytic cells greater than 50%. Clearance curves of MbB and Fluoresbrite indicated rapid disappearance of particles from the blood. Results indicate that administration of particulate suspensions is not a good method for characterizing the phag...Continue Reading

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