PMID: 590405Nov 1, 1977

Phagocytosis of 'old' red blood cells by macrophages from syngeneic mice in vitro

Experimental Hematology
A KnyszynskiD Danon


We have studied in vitro the interaction of peritoneal macrophages with 'old' and 'young' RBC, as well as with enzymatically treated 'old' and 'young' RBC from syngeneic mice. 'Old' RBC were recognized and phagocytized by macrophages, whereas 'young' RBC were not. Neuraminidase treatment of both 'young' and 'old' RBC had little effect on the extent of phagocytosis. Trypsin treatment, on the other hand, markedly reduced the phagocytosis of 'old' RBC and had no effect on the phagocytosis of 'young' RBC. The level of phagocytosis of 'old' RBC by macrophages from mineral-oil treated mouse peritoneal cavities was roughly half that of macrophages from untreated mice. It is postualted that 'old' RBC could be recognized due to the presence of cytophilic antibodies on the surface of the macrophages. The specificity of these hypothetical cytophilic antibodies is believed to be directed towards sites which are absent or shielded in 'young' RBC, and exposed in 'old' RBC. Trypsin treatment of 'old' RBC appears to remove these antigenic sites from the 'old' RBC. The lower level of phagocytosis of 'old' RBC by mineral-oil induced macrophages could be due to the previous phagocytic activity of these cells, and their relatively uncoated, newly ...Continue Reading

Related Concepts


Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.