Pharmaceutical applications of isoelectric focusing on microchip with imaged UV detection

Journal of Chromatography. a
Markéta VlckováMaria A Schwarz

Abstract

For the first time, the application of a commercial Shimadzu microchip electrophoresis system MCE-2010 equipped with an imaging UV detector for isoelectric focusing (IEF) of therapeutic proteins is reported. By proper adjustment of the pH gradient, samples with pI values ranging from 2.85 to 10.3 can be focused to the imaged part of the separation channel. Three therapeutic proteins (hirudin, erythropoietin, and bevacizumab) have been successfully focused on the microchip, and the results have been compared to conventional capillary IEF in terms of peak profile, pI values, and reproducibility.

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Citations

Aug 12, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Fumihiko KitagawaKoji Otsuka
May 9, 2015·Journal of Chromatography. a·Nina NordmanTiina Sikanen
Jul 28, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Manuel GregoritzaFerdinand P Brandl
Oct 15, 2013·Electrophoresis·Shuai Sherry Zhao, David D Y Chen
Dec 25, 2008·Electrophoresis·Kiyohito Shimura
Mar 5, 2009·Electrophoresis·Greg J Sommer, Anson V Hatch
Mar 2, 2017·Proteomes·Melissa R Pergande, Stephanie M Cologna
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Jan 16, 2009·Analytical Chemistry·Nathan A MarineJonathan D Posner
Nov 9, 2010·Bioconjugate Chemistry·C Andrew BoswellLeslie A Khawli
Aug 13, 2010·Journal of Chromatography. a·Agnes VoitlMassimo Morbidelli

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