PMID: 11934230Apr 6, 2002Paper

Pharmaceutical evaluation of gas-filled microparticles as gene delivery system

Pharmaceutical Research
Stefanie SeemannRegina Reszka

Abstract

To produce and characterize a nonviral ultrasound-controlled release system of plasmid DNA (pDNA) encapsulated in gas-filled poly(D,L-lactide-co-glycolide) microparticles (PLGA-MPs). Different cationic polymers were used to form pDNA/polymer complexes to enhance the stability of pDNA during microparticle preparation. The physico-acoustical properties of the microparticles, particle size, pDNA integrity, encapsulation efficiency and pDNA release behavior were studied in vitro. The microparticles had an average particle size of around 5 microm. More than 50% of all microparticles contained a gas core, and when exposed to pulsed ultrasound as used for color Doppler imaging create a signal that yields typical color patterns (stimulated acoustic emission) as a result of the ultrasound-induced destruction of the microparticles. Thirty percent of the pDNA used was successfully encapsulated and approximately 10% of the encapsulated pDNA was released by ultrasound within 10 min. Plasmid DNA can be encapsulated in biodegradable gas-filled PLGA-MPs without hints for a structural disintegration. A pDNA release by ultrasound-induced microparticle-destruction could be shown in vitro.

Citations

May 5, 2007·Expert Opinion on Biological Therapy·Ernst Wagner
Mar 28, 2007·Pharmaceutical Research·Verena Russ, Ernst Wagner
Sep 27, 2008·Expert Opinion on Drug Delivery·Christian R MayerRaffi Bekeredjian
Mar 19, 2013·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·K Heath Martin, Paul A Dayton
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