Abstract
The activity of ampicillin-sulbactam against beta-lactamase-producing Escherichia coli has been questioned. Therefore, in this study, the killing activity of ampicillin-sulbactam was investigated in an in vitro infection model which simulates human pharmacokinetics. One ampicillin-sensitive strain (E. coli ATCC 25922, ampicillin-sulbactam MIC = 4/2 microg/ml) and three ampicillin-resistant TEM-1-producing strains with various levels of ampicillin-sulbactam resistance (EC11, MIC = 4/2 microg/ml; TIM2, MIC = 12/6 microg/ml; and GB85, MIC > 128/64 microg/ml) were studied. The E. coli strains were exposed to ampicillin-sulbactam at a starting inoculum of 6 to 7 log10 CFU/ml. Ampicillin-sulbactam was infused over 30 min to simulate doses of 3 and 1.5 g every 6 h for 24 h. The 3-g ampicillin-sulbactam dose was bactericidal against E. coli ATCC 25922, EC11, and TIM2. The 1.5-g dose displayed bactericidal activity against ATCC 25922 and EC11 similar to that of the higher dose but failed to kill TIM2 due to inadequate time above the MIC and increased MICs over 24 h. GB85 was highly resistant and grew similarly to controls. Despite an MIC at 10(7) CFU/ml indicating resistance (20/10 microg/ml), TIM2 was killed by the 3-g dose of ampicill...Continue Reading
References
Sep 1, 1978·Antimicrobial Agents and Chemotherapy·A R EnglishW E Barth
Nov 11, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C C Sanders, W E Sanders
Dec 1, 1992·Antimicrobial Agents and Chemotherapy·B J McGrathM J Rybak
Jan 1, 1992·Gynecologic and Obstetric Investigation·M C MaberryL C Gilstrap
Feb 1, 1989·The Journal of Infectious Diseases·J E LeggettW A Craig
Nov 1, 1986·Reviews of Infectious Diseases·G Foulds
Jan 1, 1988·Drugs·M A Castellano
Jan 1, 1988·Drugs·M F Güneren
Oct 1, 1988·The Journal of Infectious Diseases·B VogelmanW A Craig
Mar 1, 1980·The Journal of Antimicrobial Chemotherapy·R WiseK A Bedford
Apr 1, 1995·Antimicrobial Agents and Chemotherapy·P D Lister, C C Sanders
May 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M A PfallerJ C McLaughlin
Feb 1, 1993·Antimicrobial Agents and Chemotherapy·P A Bradford, C C Sanders
Mar 1, 1993·Antimicrobial Agents and Chemotherapy·L B RiceD M Shlaes
May 1, 1997·Diagnostic Microbiology and Infectious Disease·R N Jones, M N Dudley
Citations
Jul 22, 2019·Clinical Pharmacokinetics·Chandra Datta SumiFekade B Sime
Jul 22, 2009·Antimicrobial Agents and Chemotherapy·Kerry L LaPlante, George Sakoulas
Jul 1, 2020·Expert Review of Anti-infective Therapy·Sofie DhaeseJan De Waele
Jul 28, 2020·Clinical Pharmacokinetics·Sofie DhaeseJan J De Waele
Nov 21, 2001·Pharmacotherapy·B W GundersonJ C Rotschafer