Pharmacokinetic Evaluation of Metabolic Drug Interactions between Repaglinide and Celecoxib by a Bioanalytical HPLC Method for Their Simultaneous Determination with Fluorescence Detection

Pharmaceutics
Dong-Gyun HanIn-Soo Yoon

Abstract

Since diabetes mellitus and osteoarthritis are highly prevalent diseases, combinations of antidiabetic agents like repaglinide (REP) and non-steroidal anti-inflammatory drugs (NSAID) like celecoxib (CEL) could be commonly used in clinical practice. In this study, a simple and sensitive bioanalytical HPLC method combined with fluorescence detector (HPLC-FL) was developed and fully validated for simultaneous quantification of REP and CEL. A simple protein precipitation procedure and reversed C18 column with an isocratic mobile phase (mixture of ACN and pH 6.0 phosphate buffer) were employed for sample preparation and chromatographic separation. The fluorescence detector was set at a single excitation/emission wavelength pair of 240 nm/380 nm. The linearity (10-2000 ng/mL), accuracy, precision, extraction recovery, matrix effect, and stability for this method were validated as per the current FDA guidance. The bioanalytical method was applied to study pharmacokinetic interactions between REP and CEL in vivo, successfully showing that concurrent administration with oral REP significantly altered the pharmacokinetics of oral CEL. Furthermore, an in vitro metabolism and protein binding study using human materials highlighted the poss...Continue Reading

References

Jan 30, 2002·Biomedical Chromatography : BMC·Ulrike WernerKay Brune
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Elke StörmerIvar Roots
Aug 16, 2003·British Journal of Clinical Pharmacology·Tanja Busk BidstrupKristian Tage Hansen
Mar 18, 2004·British Journal of Clinical Pharmacology·Mikko NiemiPertti J Neuvonen
May 13, 2004·Journal of Pharmaceutical and Biomedical Analysis·Hassan JalalizadehAbbas Shafiee
Jan 24, 2007·Journal of Pharmaceutical and Biomedical Analysis·Abu Bakar RuzilawatiSiew Hua Gan
Jan 1, 2008·Arthritis and Rheumatism·Reva C LawrenceUNKNOWN National Arthritis Data Workgroup
Sep 21, 2010·Joint, Bone, Spine : Revue Du Rhumatisme·Jean-Frédéric BoyerArnaud Constantin
Jun 24, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Brooke M VandenBrinkJan L Wahlstrom
Feb 18, 2012·Pharmacogenetics and Genomics·Li GongTeri E Klein
May 29, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pavel PtáčekJan Macek
Jul 10, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mi-Sun ParkKyung-Tae Lee
Dec 3, 2014·Clinics in Geriatric Medicine·Sara R PivaFrederico G S Toledo
Dec 8, 2014·Best Practice & Research. Clinical Rheumatology·Lyn MarchAnthony D Woolf
Aug 19, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yong MaMing Hu
Jun 5, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yunting XuHongjian Zhang
Jun 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sang-Bum KimIn-Soo Yoon
Nov 23, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Sang-Bum KimIn-Soo Yoon

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Methods Mentioned

BETA
equilibrium dialysis

Software Mentioned

GraphPad Prism
GraphPad
LC Solution
WinNonlin

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