Pharmacokinetic study of liposome-encapsulated human interferon-gamma after intravenous and intramuscular injection in mice

Journal of Interferon Research
I RutenfranzH Kirchner

Abstract

We encapsulated human interferon-gamma (HuIFN-gamma) into liposomes and analyzed whether this preparation prevented the rapid decay of IFN-gamma in the serum of C57BL/6 mice after intravenous or intramuscular injection. Furthermore, we compared the serum decay curve of liposomal and free IFN-gamma. Whereas the intramuscular injection of IFN-gamma resulted in a serum curve with entrance compartment and subsequent biphasic elimination, intravenously injected IFN-gamma was distributed and eliminated in a biphasical manner from serum. Extremely prolonged serum titers are caused by IFN-gamma liposomes with a phospholipid composition of dimyristoylphosphatidylcholine/cholesterol/dicetylphosphate and an average particle size of 333 nm. Intramuscular injection of 2.5 mumoles liposome suspension with an antiviral activity of 4.3 x 10(3) IU/mouse resulted in longer-lasting serum titers than intravenously injected liposomes of a different charge with 2.5 mumoles and 1.2 x 10(5) IU/mouse. Liposomes after intravenous injection could be detected for up to 62 h at a titer of 20 IU/ml serum. Intramuscularly injected liposomes of the lower activity still had a titer of 30-80 IU/ml after 80 h p.i.

References

Feb 1, 1986·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S Vadhan-RajS E Krown
Dec 1, 1986·Journal of Interferon Research·K CantellA De Reus
Oct 1, 1988·Journal of Interferon Research·I Rutenfranz, H Kirchner
Jul 1, 1986·Annals of Internal Medicine·G Lopez-Berestein
Feb 1, 1982·Journal of Virology·D A Eppstein, W E Stewart
Jan 1, 1982·Journal of Interferon Research·D A Eppstein

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Citations

Apr 1, 1991·Current Opinion in Biotechnology·L Illum, S S Davis
Sep 13, 2007·Critical Reviews in Biotechnology·Mehrdad HamidiMahshid Foroozesh

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