Pharmacokinetics and cytotoxic study of euphol from Euphorbia umbellata (Bruyns) Pax latex

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Luiza S CruzFlávio Luis Beltrame

Abstract

Medicinal plants are an important source to identify new active pharmaceutical compounds. Traditionally, the sap of Euphorbia umbellata is widely used to treat cancer and inflammatory conditions. These effects have been attributed to the presence of terpenes and phenolic compounds in the extracts of this plant. Euphol, a tetracyclic triterpene alcohol, is one of the major compounds present in Euphorbia species, and some biological activities have been attributed to this compound. This study aimed to evaluate the in vitro cytotoxicity of euphol against Jurkat, HL-60, K-562, B16F10, and HRT-18 cells lines, as well as the biological stability, distribution, metabolism properties in vitro, and the determination of the concentration of euphol in the plasma and liver of rats. The MTT reduction assay was used to evaluate the cytotoxicity of euphol against cancer cell lines, and the selectivity index, the morphology and cell cycle assays to evaluate the death mechanisms in K-562 and B16F10 lineages. UHPLC-MS was applied for the in vivo evaluation of the concentration of euphol in plasma and liver, and in vitro metabolic stability in human liver microsomes and S9 fraction, plasma protein binding, and stability in simulated gastric and i...Continue Reading

Citations

Apr 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elaine C D GonçalvesRafael C Dutra
Jun 11, 2021·Life Sciences·Thais Latansio de OliveiraFlávio Luís Beltrame
Aug 28, 2021·ACS Synthetic Biology·Weibo QiaoYansheng Zhang

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