Pharmacokinetics and excretion study of bergenin and its phase II metabolite in rats by liquid chromatography tandem mass spectrometry

Biomedical Chromatography : BMC
Yanping RenLantong Zhang

Abstract

A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the determination of bergenin and its phase II metabolite in rat plasma, bile and urine has been developed. Biological samples were pretreated with protein precipitation extraction procedure and enzymatic hydrolysis method was used for converting glucuronide metabolite to its free form bergenin. Detection and quantitation were performed by MS/MS using electrospray ionization and multiple reaction monitoring. Negative electrospray ionization was employed as the ionization source. Sulfamethoxazole was used as the internal standard. The separation was performed on a reverse-phase C18 (250 × 4.6 mm, 5 μm) column with gradient elution consisting of methanol and 0.5% aqueous formic acid. The concentrations of bergenin in all biological samples were in accordance with the requirements of validation of the method. After oral administration of 12 mg/kg of the prototype drug, bergenin and its glucuronide metabolite were determined in plasma, bile and urine. Bergenin in bile was completely excreted in 24 h, and the main excreted amount of bergenin was 97.67% in the first 12 h. The drug recovery in bile within 24 h was 8.97%. In urine, the main...Continue Reading

References

Mar 28, 2003·Bioorganic & Medicinal Chemistry·Hironobu TakahashiYoshiyasu Fukuyama
Apr 4, 2006·Biomedical Chromatography : BMC·Yan-Bin ShiQing-Gang Meng
Aug 4, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei ZhangYung-Chi Cheng
Feb 13, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Maho Sumiyoshi, Yoshiyuki Kimura

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