Abstract
Coffee is widely consumed worldwide; therefore, the methylxanthines contained in coffee, mainly caffeine (CF), are among the most abundant bioactive compounds in our diet. In the present work, the bioavailability and metabolism of methylxanthines in a commercial soluble green/roasted coffee blend was studied. After a 3-day restriction of methylxanthine-containing foods, fasting healthy subjects (12 men and women) consumed the coffee product containing 70.69mg CF and 0.119mg theobromine (TB). Plasma samples were taken before (t=0h) and after coffee consumption at different time points (0.5, 1, 1.5, 2, 3, 4, 5, 6, 8, 9, 10 and 12h). Urine was collected at baseline (-2-0h) and at different intervals (0-2, 2-5, 5-8, 8-12 and 12-24h). Samples were analyzed by HPLC-DAD and LC-MS-QToF, and pharmacokinetic parameters were calculated. CF was the main methylxanthine found in plasma (Cmax=10.50μM, Tmax=1.2h). In addition, seven methylxanthines and methyluric acids were detected between 0.5 and 12h after coffee intake, paraxanthine (PX) being the major metabolite (Cmax=3.36μM), followed by 1-methyluric acid (1-MU; Cmax=1.44μM) and 1-methylxanthine (1-MX; Cmax=1.27μM), identified in plasma samples for the first time. In 24h urine, eleven me...Continue Reading
Citations
Sep 9, 2015·Analytical and Bioanalytical Chemistry·Omar AlharbiRoyston Goodacre
Mar 14, 2019·Journal of Medicinal Food·Henrique BossoAdriano Cressoni Araújo
Dec 12, 2019·Critical Reviews in Food Science and Nutrition·Dinh-Chuong PhamBharath Kumar Velmurugan
Feb 12, 2020·Molecular Nutrition & Food Research·Edith VisserBaukje de Roos
Oct 16, 2016·European Journal of Nutrition·Beatriz SarriáLaura Bravo-Clemente
Apr 26, 2019·European Journal of Clinical Nutrition·Gerard Ngueta
May 4, 2018·Journal of the American College of Nutrition·Nilüfer Acar-TekBüşra Ayhan
Apr 7, 2018·Critical Reviews in Food Science and Nutrition·João MonteiroBranca M Silva
Feb 24, 2017·Food & Function·Gema BaezaBaukje de Roos
Feb 23, 2020·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Xiaohua ZhaoLing Yang
Dec 11, 2020·Molecular Nutrition & Food Research·Claudia FavariDaniele Del Rio
Jan 4, 2021·Journal of Environmental Management·S KeerthananMeththika Vithanage
Nov 13, 2020·Toxicology Research·Débora F GonçalvesNélson R Carvalho
Dec 31, 2020·Antioxidants·Rozalia-Maria AnastasiadiMarina Resmini
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wiem Haj AhmedChristian Carpéné
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nuntouchaporn HutachokSomdet Srichairatanakool
Aug 9, 2021·Talanta·Bassam LajinBarbara Obermayer-Pietsch
May 28, 2019·Chemical Research in Toxicology·Zuzana JandovaChris Oostenbrink
Jun 27, 2020·Nutrition Research·Rômulo P BarcelosLuiz Ff Royes
Apr 25, 2015·Journal of Agricultural and Food Chemistry·Naira Poerner RodriguesNeura Bragagnolo
May 28, 2021·Experimental Animals·Dina Villanueva-GarcíaIsmael Hernández-Avalos