Pharmacologic concentrations of ascorbic acid cause diverse influence on differential expressions of angiogenic chemokine genes in different hepatocellular carcinoma cell lines

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Zu-Yau Lin, Wan-Long Chuang

Abstract

This study was to investigate whether ascorbic acid (AA) at pharmacologic concentration became prooxidant and had the potential to influence the expressions of angiogenic and angiostatic chemokine genes in hepatocellular carcinoma (HCC) cell lines. Influence of low (1 mM) and high (30 mM) pharmacologic concentrations of AA on two HCC cell lines (cell line A, HCC24/KMUH; cell line B, HCC38/KMUH) were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Three angiogenic genes (CCL2, CXCL6, IL8), one angiostatic gene (CXCL10) and two genes related to oxidative stress (SOD2, VNN3) were selected for quantitative RT-PCR study. Both low and high pharmacologic concentrations of AA up-regulated CCL2, CXCL6, IL8, SOD2 and VNN3 genes in cell line A, but down-regulated CCL2 and IL8 genes in cell line B. CXCL6 gene in cell line B was down-regulated by high pharmacologic concentration of AA. CXCL10 gene was up-regulated by low pharmacologic concentration of AA, but was down-regulated by high pharmacologic concentration of AA in both cell lines. Low pharmacologic concentration of AA up-regulated VNN3 gene and high pharmacologic concentrat...Continue Reading

References

Nov 10, 1995·The Journal of Biological Chemistry·R M StrieterD Marriott
Oct 25, 2002·International Journal of Cancer. Journal International Du Cancer·Masahiro OhtaKazuaki Chayama
Jul 30, 2004·Molecular and Cellular Biology·C BerruyerF Galland
Nov 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ina H BenoyLuc Y Dirix
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Qi ChenMark Levine
Sep 5, 2006·Apoptosis : an International Journal on Programmed Cell Death·Prachee GokhaléMargret C M Vissers
Jan 24, 2007·Cancer Letters·Yoshiya TachibanaShuichi Kaneko
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Qi ChenMark Levine
Mar 18, 2008·Journal of Applied Toxicology : JAT·Nuria ArranzPaloma Morales
Aug 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qi ChenMark Levine
Mar 6, 2009·European Journal of Clinical Investigation·Z Y LinY H Chuang

❮ Previous
Next ❯

Citations

Apr 30, 2013·Antioxidants & Redox Signaling·Nermi L ParrowMark Levine
Jun 12, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zu-Yau Lin, Wan-Long Chuang
Jun 29, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zu-Yau LinWan-Long Chuang
Oct 24, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zu-Yau Lin, Wan-Long Chuang
Mar 16, 2011·Free Radical Biology & Medicine·Michael Graham EspeyQi Chen
Oct 3, 2013·Molecular Immunology·Matheus Fernandes Costa-SilvaAndréa Teixeira-Carvalho
Jun 1, 2019·Medical Anthropology·Tirsa Colmenares-Roa, Ingris Peláez-Ballestas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.