PMID: 15223802Jun 30, 2004Paper

Pharmacologic disruption of Schlemm's canal cells and outflow facility in anterior segments of human eyes

Investigative Ophthalmology & Visual Science
Cindy BahlerDouglas H Johnson

Abstract

To determine the effect of disruption of Schlemm's canal cells on outflow facility. Pharmacologic agents that weaken the cytoskeleton or interfere with integrin binding may allow targeted disruption of the cells lining Schlemm's canal because of the transmural pressure gradient the cells face as aqueous passes into the canal. Anterior segments of human eyes were placed in perfusion organ culture, and either single or sequential doses of H-7 or RGD peptide were added. Fellow eyes received vehicle or RGE peptide. Eyes were fixed and examined by light and electron microscopy. Both agents caused a partial loss of the endothelial lining of Schlemm's canal cells without disruption of trabecular cells in other regions. H-7 significantly increased outflow facility after single or sequential doses, with moderate cell loss of both the inner and outer wall canal cells: 20.0% +/- 10.5% of the width of the canal versus 5.2% +/- 3.7% in control meshworks (P = 0.05). No significant correlation between the amount of canal cell loss and outflow facility was found. RGD was associated with a variable loss of canal cells but did not change outflow facility. Pharmacologic disruption of Schlemm's canal cells appears possible. H-7 increased outflow f...Continue Reading

Citations

Jan 21, 2012·Current Opinion in Ophthalmology·W Daniel Stamer, Ted S Acott
Aug 8, 2013·Investigative Ophthalmology & Visual Science·Chen-Yuan Charlie YangHaiyan Gong
Nov 26, 2013·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Debjani GagenDonna M Peters
Nov 9, 2007·American Journal of Ophthalmology·Cindy K BahlerDouglas H Johnson
Oct 19, 2005·Biochemical and Biophysical Research Communications·Paloma B LitonPedro Gonzalez
Mar 4, 2008·Experimental Eye Research·Ted S Acott, Mary J Kelley
Mar 1, 2006·The Journal of Gene Medicine·Teresa BorrásSarah S Chisolm
Jul 1, 2009·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Marc D Jacobs
Jun 28, 2012·Expert Review of Ophthalmology·Baohe Tian, Paul L Kaufman
Dec 19, 2017·Experimental and Therapeutic Medicine·Lin CongYuyan Zhang
Jan 14, 2021·Scientific Reports·Ravinarayanan HaribalaganeshSrinivasan Senthilkumari
Nov 18, 2005·Experimental Eye Research·Shannon M ConleyW Daniel Stamer

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