Pharmacological characterization and tissue distribution of the human and rat GALR1 receptors

Biochemical and Biophysical Research Communications
K A SullivanM A Cascieri

Abstract

The diverse biological functions of galanin are mediated via membrane bound high-affinity receptors. In order to identify and characterize potential galanin receptor subtypes, we have examined the specific 125I-galanin binding to the CHP-212 human neuroblastoma cell line. The galanin receptors expressed in CHP-212 cells, like GALR1 have high affinity for galanin (Kd = 0.07 nM) and the potency for inhibition of 125I-galanin binding by galanin peptides parallels that of hGALR1 expressed in a stable CHO cell line. We confirmed that GALR1 is expressed in these cells by RT-PCR. We further determined the tissue expression patterns of hGALR1 which is expressed in a variety of human tissues at a very low level, with the highest levels seen in heart, small intestine and prostate. A species of approximately 70 kDa is recognized by antisera specific for hGALR1 by Western blot analysis and should allow future measurements of receptor protein expression.

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Citations

Feb 3, 1999·Annals of the New York Academy of Sciences·T P IismaaJ Shine
Feb 5, 2013·Life Sciences·Penghua FangMingyi Shi
Oct 28, 2010·General and Comparative Endocrinology·John Chi Wang HoFrederick C Leung
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