Pharmacological characterization of kinin-induced relaxation of human corpus cavernosum

British Journal of Urology
C E TeixeiraGilberto De Nucci

Abstract

To characterize the kinin receptor subtype involved in the relaxation of human isolated corpus cavernosum (HCC) induced by bradykinin (BK), Lys-bradykinin (Lys-BK), Met-Lys-bradykinin (Met-Lys-BK) and des-Arg9-bradykinin, and to investigate whether the kinin-induced relaxation of HCC results from the stimulation of nonadrenergic, noncholinergic (NANC) neurons supplying the cavernosal tissue. Excised HCC tissues were immediately placed in Krebs solution and kept at 4 degrees C until use (never > 24 h after removal). HCC was cut in strips of approximately 2 cm, suspended in a cascade system and superfused with oxygenated and warmed Krebs solution at 5 mL/min. After equilibration for approximately 90 min, noradrenaline (3 micromol/L) was infused to induce a submaximal contraction of the HCC strips. The release of cyclo-oxygenase products was prevented by infusing indomethacin (6 micromol/L). HCC strips were calibrated by injecting a single bolus of the nitrovasodilator glyceryl trinitrate (GTN) and the sensitivity of the tissues adjusted electronically to be similar. The agonists (kinins, histamine and acetylcholine) were injected as a single bolus (up to 100 microL) and the relaxation of HCC expressed as a percentage of the subma...Continue Reading

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Citations

Mar 30, 2010·International Journal of Andrology·T WangZ Q Ye
Jun 10, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Justin A EdwardPhilip J Kadowitz
Apr 2, 2003·Expert Opinion on Therapeutic Targets·Liming JinR Clinton Webb
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Oct 16, 2008·Journal of Hypertension·Athanasios Manolis, Michael Doumas
Mar 11, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Carolina MondilloRamiro Vázquez

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