PMID: 8606794Nov 1, 1995Paper

Pharmacological characterization of presynaptic calcium channels using subsecond biochemical measurements of synaptosomal neurosecretion

Neuropharmacology
T J Turner, K Dunlap

Abstract

The recent development of peptide antagonists that selectively block subtypes of neuronal calcium channel has provided tools to study the role of presynaptic calcium channels in triggering exocytosis. A variety of methods have consistently demonstrated that multiple channel types participate in exocytosis. We have studied the subsecond kinetics of [3H]glutamate release from rat cortical synaptosomes as an assay for presynaptic calcium channel activity. The system has been characterized over a broad range of conditions in an effort to compare biochemical measurements of transmitter release with electrophysiological measurements of synaptic currents. The efficacies of omega-agatoxin IVA and omega-conotoxins GVIA and MVIIC were increased when Ca2+ influx was decreased by: (1) decreasing the KCl concentration to diminish the extent of depolarization, (2) decreasing the Ca2+ concentration, or (3) partially blocking Ca2+ influx with one of the other antagonists. By using these toxins in combination, we found that at least three types of pharmacologically distinct channel participate in exocytosis. The largest fraction of glutamate release is blocked by omega-agatoxin IVA (IC50 = 12.2 nM) and by omega-conotoxin MVIIC (IC50 = 35 nM), c...Continue Reading

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Citations

Feb 5, 2000·European Journal of Pharmacology·G J SangerC D Benham
Dec 2, 2000·Neurochemistry International·D D SavageA M Allan
Dec 31, 1997·Neurochemistry International·K Langley, N J Grant
Dec 25, 2002·Progress in Neurobiology·Luca RaiteriGiambattista Bonanno
Aug 28, 1999·Progress in Neurobiology·M R Bennett
Jun 1, 1996·Current Opinion in Neurobiology·H Reuter
Nov 14, 1997·British Journal of Pharmacology·C GuatimosimM A Prado
Aug 24, 1999·The European Journal of Neuroscience·E A Lukyanetz, E Neher
Aug 13, 2008·Neuroreport·Su-Jane WangChia-Yu Huang
Jan 8, 1998·The European Journal of Neuroscience·E Vázquez, J Sánchez-Prieto
Feb 28, 2002·Molecular and Cellular Biology·Atsuko PolzinTimothy J Turner
Apr 15, 1999·Annual Review of Neuroscience·A B MacDermottS A Siegelbaum
Mar 15, 2015·International Journal of Molecular Sciences·Tzu-Yu LinSu-Jane Wang
Apr 3, 2013·Biochemical and Biophysical Research Communications·Xiaoli TianEiki Takahashi
Oct 17, 2012·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Maike Hartlage-RübsamenSteffen Roßner
Dec 9, 2008·Neurochemistry International·Yi Chang, Su-Jane Wang
May 30, 2008·Diabetes Research and Clinical Practice·Eiki Satoh, Ayako Takahashi
Dec 20, 2008·Journal of Neurochemistry·Carolina LaderaJosé Sánchez-Prieto
Oct 20, 2007·Journal of Neurochemistry·Carolina LaderaJosé Sánchez-Prieto

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