Pharmacological characterization of the involvement of protein kinase C in oscillatory and non-oscillatory calcium increases in astrocytes

Journal of Pharmacological Sciences
Mitsuhiro MoritaYoshihisa Kudo

Abstract

Evidence increasingly shows that astrocytes play a pivotal role in brain physiology and pathology via calcium dependent processes, thus the characterization of the calcium dynamics in astrocytes is of growing importance. We have previously reported that the epidermal growth factor and basic fibroblast growth factor up-regulate the oscillation of the calcium releases that are induced by stimuli, including glutamate in cultured astrocytes. This calcium oscillation is assumed to involve protein kinase C (PKC), which is activated together with the calcium releases as a consequence of inositol phospholipid hydrolysis. In the present study, this issue has been investigated pharmacologically by using astrocytes cultured with and without the growth factors. The pharmacological activation of PKC largely reduced the glutamate-induced oscillatory and non-oscillatory calcium increases. Meanwhile, PKC inhibitors increased the total amounts of both calcium increases without affecting the peak amplitudes and converted the calcium oscillations to non-oscillatory sustained calcium increases by abolishing the falling phases of the repetitive calcium increases. Furthermore, the pharmacological effects were consistent between both glutamate- and h...Continue Reading

References

Jan 28, 2004·Journal of Pharmacological Sciences·Mitsuhiro MoritaYoshihisa Kudo
Oct 8, 2005·Science·Olivier PascualPhilip G Haydon
Jan 10, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yuriy M UsachevStanley A Thayer
Oct 5, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shinghua DingPhilip G Haydon
Oct 17, 2008·Physiological Reviews·Susan F Steinberg
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Sep 13, 2011·Nature Neuroscience·Maria Amalia Di CastroAndrea Volterra
Feb 22, 2013·Journal of Neurophysiology·Prakash DevarajuTodd A Fiacco
Aug 15, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lydie MorelYongjie Yang

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Citations

Dec 22, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Guixian MengImshik Lee
Jan 22, 2020·Scientific Reports·Timo FischerChristian Lohr
Sep 28, 2017·Mediators of Inflammation·Julieta AlcainMónica Vermeulen

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