Abstract
1. Leukotriene B(4) (LTB(4)) stimulation of guinea-pig peritoneal eosinophils, induced a biphasic activation of the NADPH oxidase composed of a rapid (<3 min) phase mediated by non-adherent cells and a sustained (3 - 120 min) phase mediated by CD11b/CD18 adherent eosinophils. Studies were undertaken to compare the intracellular mechanism that mediate these responses. 2. SB 203580 and PP1, inhibitors of p38 mitogen-activated protein (MAP) kinase and the src-family protein tyrosine kinases, respectively caused concentration-dependent attenuation of both the rapid (SB203580: pD(2)=-6.31; PP1: pD(2)=-5.50) and sustained (SB203580: pD(2)=-6.50; PP1: pD(2)=-5.73) phases. Similarly, the MAP kinase kinase-1 inhibitor, PD098059 produced partial inhibition of the both phases of superoxide generation. 3. The protein kinase C (PKC) inhibitors Ro-31 8220, GF 109203X and Gö 6976 attenuated the rapid NADPH oxidase response (pD(2)s=-6.10, -6.72, -6.15 respectively) and, to a lesser extent, (pD(2)s=-5.54, -6.02, -6.51 respectively) the sustained phase. 4. An inhibitor of phosphatidylinositol 3-kinase (PtdIns 3-kinase), wortmannin caused concentration dependent attenuation of the sustained (pD(2)=-8.68) but not rapid phase of superoxide generati...Continue Reading
References
Jan 1, 1992·American Journal of Respiratory Cell and Molecular Biology·E M ZorattiW W Busse
Dec 1, 1987·The Journal of Clinical Investigation·C F Nathan
Dec 18, 1989·FEBS Letters·P D DavisS E Wilkinson
Nov 30, 1989·Biochemical and Biophysical Research Communications·R L Geahlen, J L McLaughlin
Mar 12, 1987·Journal of Immunological Methods·H Gyllenhammar
Mar 1, 1973·The Journal of Clinical Investigation·B M BabiorJ T Curnutte
Jan 1, 1994·Growth Factors·Q X Zhang, G S Baldwin
Jun 1, 1995·American Journal of Respiratory Cell and Molecular Biology·F L JahnsenP Brandtzaeg
Sep 15, 1995·The Biochemical Journal·R S PerkinsM A Giembycz
Aug 17, 1995·Nature·B M Burgering, P J Coffer
Dec 1, 1993·The Biochemical Journal·A Arcaro, M P Wymann
Aug 31, 1993·Biochemical and Biophysical Research Communications·S S SinghV P Chauhan
Jan 12, 1996·The Journal of Biological Chemistry·J H HankeP A Connelly
Oct 11, 1996·The Journal of Biological Chemistry·A PtasznikG M Bokoch
Oct 1, 1996·Current Biology : CB·S A DidichenkoM Thelen
Mar 27, 1997·Biochemical and Biophysical Research Communications·B MetznerJ Norgauer
Feb 3, 1998·American Journal of Respiratory Cell and Molecular Biology·G DentK F Rabe
Jun 25, 1998·Molecular and Cellular Biology·F KieferT Pawson
Jan 1, 1997·Memórias do Instituto Oswaldo Cruz·M A Lindsay, M A Giembycz
Oct 10, 1998·Journal of Leukocyte Biology·M A LindsayM A Giembycz
Jan 15, 1999·The Journal of Cardiovascular Nursing·K H Gylys
Jun 8, 1999·European Journal of Immunology·L A MillerR L Geahlen
Jun 12, 1999·The Netherlands Journal of Medicine·L Koenderman, J W Lammers
Oct 16, 1999·The Journal of Biological Chemistry·A CraxtonE A Clark
Oct 19, 1999·Biochimica Et Biophysica Acta·R LöfgrenO Stendahl
Nov 7, 1999·The Journal of Biological Chemistry·L O BeitzA M Scharenberg
Dec 1, 1999·British Journal of Pharmacology·O T LynchM A Lindsay
Mar 4, 2000·The Journal of Biological Chemistry·F V LaliB M Foxwell
Citations
Oct 3, 2003·Veterinary Immunology and Immunopathology·E C GreenawayN T Goode
Jul 10, 2004·Antioxidants & Redox Signaling·David GreggHervé Kovacic
Jun 11, 2005·International Archives of Allergy and Immunology·Myeong Heon ShinSung Ho Ryu
Jan 16, 2007·American Journal of Respiratory Cell and Molecular Biology·Xiangdong ZhuAlan R Leff
Feb 19, 2005·Blood·Carlos H C SerezaniMarc Peters-Golden
Mar 19, 2003·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Robert Newton, Neil Holden
May 11, 2012·Journal of Clinical Biochemistry and Nutrition·Masao YamasakiKazuo Nishiyama
Oct 8, 2009·Bioscience, Biotechnology, and Biochemistry·Masao YamasakiKazuo Nishiyama
Dec 18, 2015·Prostaglandins & Other Lipid Mediators·Tapan BehlAnita Kotwani
Nov 1, 2006·Allergology International : Official Journal of the Japanese Society of Allergology·Takafumi YamaguchiMasahiko Kato
Jan 1, 2006·Expert Review of Clinical Immunology·Masahiko KatoHirokazu Kimura
Sep 7, 2002·Circulation Research·Puvi N SeshiahKathy K Griendling
Mar 29, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·R M TouyzE L Schiffrin
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Paul J KinghamRichard W Costello
Sep 11, 2017·The Journal of Allergy and Clinical Immunology·Daniela J CarrollBruce S Bochner