Pharmacological comparison of LTB(4)-induced NADPH oxidase activation in adherent and non-adherent guinea-pig eosinophils

British Journal of Pharmacology
O T LynchM A Lindsay

Abstract

1. Leukotriene B(4) (LTB(4)) stimulation of guinea-pig peritoneal eosinophils, induced a biphasic activation of the NADPH oxidase composed of a rapid (<3 min) phase mediated by non-adherent cells and a sustained (3 - 120 min) phase mediated by CD11b/CD18 adherent eosinophils. Studies were undertaken to compare the intracellular mechanism that mediate these responses. 2. SB 203580 and PP1, inhibitors of p38 mitogen-activated protein (MAP) kinase and the src-family protein tyrosine kinases, respectively caused concentration-dependent attenuation of both the rapid (SB203580: pD(2)=-6.31; PP1: pD(2)=-5.50) and sustained (SB203580: pD(2)=-6.50; PP1: pD(2)=-5.73) phases. Similarly, the MAP kinase kinase-1 inhibitor, PD098059 produced partial inhibition of the both phases of superoxide generation. 3. The protein kinase C (PKC) inhibitors Ro-31 8220, GF 109203X and Gö 6976 attenuated the rapid NADPH oxidase response (pD(2)s=-6.10, -6.72, -6.15 respectively) and, to a lesser extent, (pD(2)s=-5.54, -6.02, -6.51 respectively) the sustained phase. 4. An inhibitor of phosphatidylinositol 3-kinase (PtdIns 3-kinase), wortmannin caused concentration dependent attenuation of the sustained (pD(2)=-8.68) but not rapid phase of superoxide generati...Continue Reading

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Citations

Jul 10, 2004·Antioxidants & Redox Signaling·David GreggHervé Kovacic
Jan 16, 2007·American Journal of Respiratory Cell and Molecular Biology·Xiangdong ZhuAlan R Leff
Mar 19, 2003·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Robert Newton, Neil Holden
May 11, 2012·Journal of Clinical Biochemistry and Nutrition·Masao YamasakiKazuo Nishiyama
Dec 18, 2015·Prostaglandins & Other Lipid Mediators·Tapan BehlAnita Kotwani
Nov 1, 2006·Allergology International : Official Journal of the Japanese Society of Allergology·Takafumi YamaguchiMasahiko Kato
Jan 1, 2006·Expert Review of Clinical Immunology·Masahiko KatoHirokazu Kimura
Sep 7, 2002·Circulation Research·Puvi N SeshiahKathy K Griendling
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Paul J KinghamRichard W Costello

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