Pharmacological discrimination of plasmalemmal and mitochondrial sodium-calcium exchanger in cardiomyocyte-derived H9c2 cells

Biological & Pharmaceutical Bulletin
Iyuki NamekataHikaru Tanaka

Abstract

We examined the effects of SEA0400 and CGP-37157 on the plasmalemmal Na(+)-Ca(2+) exchanger (NCX) and mitochondrial NCX using H9c2 cardiomyocytes loaded with Ca(2+)-sensitive fluorescent probes. The plasmalemmal NCX activity, which was measured as the increase in cytoplasmic Ca(2+) concentration after application of low Na(+) extracellular solution, was inhibited by SEA0400 but not by CGP-37157. The mitochondrial NCX activity, which was measured in permeabilized H9c2 cells as the decrease in mitochondrial Ca(2+) concentration after application of Ca(2+)-free extramitochondrial solution, was inhibited by CGP-37157 but not by SEA0400. These results indicate that SEA0400 and CGP-37157 act as selective inhibitors towards plasmalemmal and mitochondrial NCX, respectively, and provide pharmacological evidence that the plasmalemmal and mitochondrial NCX are distinct molecular entities.

References

Apr 1, 1993·Journal of Cardiovascular Pharmacology·D A CoxM A Matlib
Dec 15, 2000·Cell Calcium·T E GunterK Gunter
Mar 6, 2002·British Journal of Pharmacology·Hikaru TanakaKoki Shigenobu
Dec 28, 2002·Annals of the New York Academy of Sciences·Donald M Bers, Christopher R Weber
Jun 17, 2003·The Journal of Pharmacology and Experimental Therapeutics·Alexander OmelchenkoLarry V Hryshko
Nov 20, 2003·The Journal of Biological Chemistry·Xinjiang Cai, Jonathan Lytton
Dec 11, 2003·American Journal of Physiology. Renal Physiology·Ilse SmetsPaul Steels
Sep 11, 2004·Journal of Pharmacological Sciences·Takahiro Iwamoto
Nov 26, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Masao SaotomeHideharu Hayashi
Dec 16, 2004·Journal of Pharmacological Sciences·Hikaru TanakaKoki Shigenobu
Dec 8, 2005·Canadian Journal of Physiology and Pharmacology·Yasushi SakaiTakahiro Iwamoto
Mar 8, 2006·World Journal of Gastroenterology : WJG·Byung Joo KimKi Whan Kim
Jul 18, 2006·European Journal of Pharmacology·Iyuki NamekataKoki Shigenobu
Apr 21, 2007·Annals of the New York Academy of Sciences·L A VenetucciD A Eisner
Jan 4, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Iyuki NamekataHikaru Tanaka
Dec 20, 2008·Archives of Biochemistry and Biophysics·Pulak KarSajal Chakraborti
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Raz PaltyIsrael Sekler
Nov 3, 2010·Methods in Cell Biology·Donald M BersRichard Nuccitelli

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Citations

Oct 9, 2020·Biological & Pharmaceutical Bulletin·Iyuki NamekataHikaru Tanaka
Jan 26, 2020·Cell Calcium·Simona MagiVincenzo Lariccia

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