PMID: 9416763Jan 1, 1997Paper

Pharmacological evidence for the contribution of polyamines as mediators of the transcriptional component involved in smooth muscle relaxation elicited by forskolin

Life Sciences
M P RevueltaA Hidalgo

Abstract

To study whether cAMP-dependent transcriptional effect and polyamines might play a modulatory role on smooth muscle, the effect of forskolin on KCl (60 mM)-induced contractions in isolated rat uterus and its modification by inhibitors of cAMP-dependent protein kinase (PKA) (Rp-cAMPS and TPCK), transcription (actinomycin D), protein synthesis (cycloheximide) and ornithine decarboxylase (alpha-difluoromethyl-ornithine, DFMO), and a polyamine (spermine) have been assayed. Forskolin (0.1 to 6 microM) induced concentration-dependent relaxation on KCl-induced tonic contractions in rat uterus (IC50: 0.55 +/- 0.12 microM) which was antagonized (p<0.05) by Rp-cAMPS (30 microM), TPCK (3 microM), cycloheximide (300 microM), actinomycin D (4 and 12 microM) and TPCK (3 microM) plus actinomycin D (12 microM). The IC50 values of forskolin in the presence of these drugs were 3.75 +/- 1.53 microM, 12.08 +/- 8.18 microM, 6.88 +/- 5.02 microM, 3.80 +/- 2.35 and 5.31 +/- 2.80 microM, and 4.26 +/- 3.65 microM respectively. Furthermore, DFMO (10 mM) also shifted the relaxation curve to forskolin to the right (IC50: 3.06 +/- 2.66 microM, p<0.05) but DFMO (10 mM) plus actinomycin D (12 microM) (IC50: 1.78 +/- 1.33 microM) did not. However, DFMO (10 mM...Continue Reading

References

Feb 18, 1992·European Journal of Pharmacology·A Abe, H Karaki
Jan 1, 1992·Pharmacology & Therapeutics·P P McCann, A E Pegg
Oct 1, 1992·Trends in Biochemical Sciences·M Karin, T Smeal
Aug 7, 1992·Cell·T Hunter, M Karin
Jan 1, 1990·Pharmacology & Therapeutics·K J Murray
Jan 1, 1991·Methods in Enzymology·A Laurenza, K B Seamon
Jan 1, 1988·Life Sciences·H Karaki, G B Weiss
Jan 1, 1988·Methods in Enzymology·L H BotelhoB Jastorff
Mar 1, 1986·The Biochemical Journal·A E Pegg
Nov 1, 1982·The American Journal of Physiology·A E Pegg, P P McCann
Jan 1, 1995·Annual Review of Pharmacology and Toxicology·L J Marton, A E Pegg
Sep 1, 1994·Naunyn-Schmiedeberg's Archives of Pharmacology·M L CandenasJ D Martín
Oct 1, 1994·Pharmacology & Therapeutics·T Godfraind
Jul 1, 1994·Trends in Pharmacological Sciences·D R Edwards
Jan 1, 1994·Annual Review of Physiology·S H Francis, J D Corbin
Jan 1, 1993·Life Sciences·J A Sánchez AparicioB Cantabrana
Jan 1, 1993·The American Journal of Physiology·S Wray
Mar 1, 1994·Trends in Endocrinology and Metabolism : TEM·C SchöflG Brabant

❮ Previous
Next ❯

Citations

Sep 17, 2003·Cardiovascular Radiation Medicine·Rajbabu Pakala
Apr 23, 2002·American Journal of Obstetrics and Gynecology·Diarmaid D HoulihanJohn J Morrison
Jul 15, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Víctor Manuel Muñoz-PérezMario I Ortiz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.