Pharmacological evidence for two types of postsynaptic glycinergic receptors on the Mauthner cell of 52-h-old zebrafish larvae

Journal of Neurophysiology
P Legendre

Abstract

The presence of homooligomeric and heterooligomeric glycine receptors (GlyRs) on the Mauthner (M) cell in the isolated medulla of 52-h-old zebrafish larvae was investigated by analysis of the effects of picrotoxin on glycine-gated channels and on glycinergic miniature inhibitory postsynaptic currents (mIPSCs). Two functionally different GlyRs have been previously described on the M cell. The effects of picrotoxin on these two GlyRs were first analyzed by measuring the relative change in their total open probability (NP(o)) with picrotoxin concentration. Picrotoxin had no significant effect on the glycine channel with a single conductance level of 40-46 pS. In contrast, picrotoxin application decreased the NP(o) of the GlyR with multiple subconductance levels. On this GlyR, picrotoxin decreased in a concentration-dependent manner the occurrence of the 80- to 86-pS substate (median inhibiting concentration = 0.89 microM) and had no apparent effect on the 40- to 46-pS opening probability. Opening frequency and the mean open times of the 80- to 88-pS main conductance state were reduced in the presence of 10 microM picrotoxin, but their relative weight remained unchanged. These effects of picrotoxin were not voltage dependent. Picro...Continue Reading

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Citations

Sep 24, 2004·Physiological Reviews·Joseph W Lynch
Aug 20, 2005·The Journal of Biological Chemistry·Rebecca Hawthorne, Joseph W Lynch
Mar 6, 2002·Journal of Neurophysiology·Siriporn C Chattipakorn, Lori L McMahon
Aug 17, 2002·The Journal of Pharmacology and Experimental Therapeutics·Gilles Martin, George Robert Siggins
Jun 7, 2007·Visual Neuroscience·Sriparna MajumdarHeinz Wässle
Jun 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hiromi HirataJohn Y Kuwada
Sep 19, 2002·Molecular Pharmacology·Pascal LegendreAntoine Triller
Jan 13, 2015·The Journal of Biological Chemistry·Suzanne ScottAngelo Keramidas
Nov 2, 2017·The Journal of General Physiology·Giovanni Gonzalez-GutierrezClaudio Grosman
Jul 29, 2020·Nature Communications·Arvind KumarSudha Chakrapani
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Jun 14, 2006·Journal of Pharmacological and Toxicological Methods·Chuenlei ParngPatricia McGrath

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