Pharmacological Inhibition of Protein Kinase C Reduces West Nile Virus Replication

Viruses
Ana B BlázquezJuan-Carlos Sáiz

Abstract

Flaviviruses are relevant animal and human pathogens that include West Nile virus (WNV), Japanese encephalitis virus, dengue virus, or Zika virus, among others. Currently, no licensed therapy is available to fight flaviviral infections. Protein kinases C (PKCs) constitute a family of multifunctional lipid-dependent isoenzymes that regulate a wide variety of cellular processes (apoptosis, differentiation, proliferation, cellular transformation, motility, adhesion, etc.) being currently considered at the front line of drug development for the treatment of diverse human disorders. PKCs have also been implicated in different steps during viral replication; however, nowadays, results regarding their role in flavivirus replication are controversial. Here we demonstrate that calphostin C and chelerythrine, two broad-PKC inhibitors that target conventional, novel and atypical PKCs, significantly inhibit WNV multiplication in cell culture without affecting cell viability. A reduction of viral yields was observed in treated cells when compared with mock-treated cells. Likewise, immunofluorescence detection of viral enveloped E protein was reduced in treated cells, as was the amount of viral RNA released to the supernatant, mainly in thos...Continue Reading

References

Nov 15, 1990·Biochemical and Biophysical Research Communications·J M HerbertJ P Maffrand
Mar 13, 1986·Biochemical and Biophysical Research Communications·T TamaokiF Tomita
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·B P DeA K Banerjee
May 1, 1997·Virus Research·O V Morozova Kit YYa
Apr 29, 2000·Trends in Pharmacological Sciences·K J WayG L King
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M MonickG Hunninghake
Jan 3, 2003·Apoptosis : an International Journal on Programmed Cell Death·C Brodie, P M Blumberg
Sep 2, 2008·Virology·Dipankar BhattacharyaRob Striker
Oct 17, 2008·Physiological Reviews·Susan F Steinberg
Apr 22, 2009·Cell Host & Microbe·Sonja WelschRalf Bartenschlager
Jan 23, 2010·Nature Reviews. Molecular Cell Biology·Carine RossePeter J Parker
Oct 26, 2012·Cellular and Molecular Life Sciences : CMLS·Helan Xiao, Mingyao Liu
Mar 26, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Bo ZhanZhe Zhang
Jun 26, 2013·Expert Opinion on Therapeutic Patents·M Elizabeth SobhiaAlekhya Kokkula
Jul 24, 2013·Virology Journal·Julie A KeatingRob Striker
Apr 12, 2012·World Journal of Virology·Miguel A Martín-Acebes, Juan-Carlos Saiz
Nov 10, 2013·PLoS Neglected Tropical Diseases·Ana-Belén BlázquezMiguel A Martín-Acebes
Mar 11, 2015·Journal of Cellular and Molecular Medicine·Zhenming JiangXi Chen
Mar 5, 2016·Virology Journal·Warobon NoppakunmongkolchaiRutaiwan Tohtong
Mar 26, 2016·Frontiers in Microbiology·Ana B BlázquezJuan-Carlos Saiz
Jan 12, 2017·Diabetes·Mauricio D DorfmanJoshua P Thaler
Mar 30, 2017·Antimicrobial Agents and Chemotherapy·Juan-Carlos Saiz, Miguel A Martín-Acebes
May 6, 2017·Nature Reviews. Drug Discovery·Veaceslav BoldescuChristian D Klein
May 17, 2017·Virology Journal·Leidy L GarcíaJhon C Castaño
Sep 28, 2017·International Journal of Molecular Medicine·Jian ZhangDahai Hu
Nov 3, 2017·Biological & Pharmaceutical Bulletin·Yuanzhang ZhuGuofu Zhu

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Citations

Feb 6, 2020·Expert Opinion on Drug Discovery·Alessandro SinigagliaLuisa Barzon
May 15, 2020·Clinical Microbiology Reviews·Naveen KumarBarry T Rouse
Dec 19, 2020·International Journal of Molecular Sciences·Ana-Belén Blázquez, Juan-Carlos Saiz
Mar 30, 2021·Heliyon·Shweta JakhmolaHem Chandra Jha
Apr 4, 2021·International Journal of Molecular Sciences·Alawiya ReslanGilles Gadea
Aug 18, 2021·Molecular & Cellular Proteomics : MCP·Patrick M VanderboomAkhilesh Pandey

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Datasets Mentioned

BETA
AHG23127

Methods Mentioned

BETA
Fluorescence
Infection

Software Mentioned

Leica Advanced Fluorescence
ImageJ
Adobe Photoshop
SPSS

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