Abstract
The present experiments were undertaken to elucidate the pharmacological nature of thromboxane A2/prostaglandin H2 receptor (TP)-mediated contraction in human intrapulmonary arteries. 9,11-epithio-11, 12-methano-thromboxane A2 (STA2) and (15S)-hydroxy-9 alpha, 11 alpha-(epoxymethano) prosta-5Z, 13E-dienoic acid (U46619) (TXA2 agonists) caused contractions in a concentration-dependent manner with EC50 values of 1.4 x 10(-9) M and 3.1 x 10(-9) M, respectively. S-1452 and ONO-3708 (TP receptor antagonists) concentration-dependently attenuated the STA2 (10(-8) M)-induced contraction with IC50 values of 5.8 x 10(-9) M and 4.2 x 10(-8) M, respectively. U-73122 (3 x 10(-6) M) and 2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate (3 x 10(-5) M) (phospholipase C inhibitors) significantly attenuated the STA2-induced contraction. Ca(+2)-induced contraction in the presence of STA2 (10(-8) M) in Ca(+2)-free medium was attenuated by nifedipine (10(-6) M) by 40%. The remaining nifedipine-resistant Ca(+2)-induced contraction was not attenuated by nitroglycerin (10(-5) M), but forskolin (10(-5) M) (adenylate cyclase stimulant) significantly decreased it by 75%. The results clearly indicate that in human intrapulmonary artery, there are TP receptors...Continue Reading
References
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M HambergB Samuelsson
Jan 1, 1992·Life Sciences·K KurahashiH Jino
Sep 30, 1992·Biochemical and Biophysical Research Communications·T YamagishiN Taira
Sep 1, 1991·Medicinal Research Reviews·S E Hall
Jan 1, 1990·Pharmacology·M A GouwP A van Zwieten
Jun 1, 1987·Circulation Research·G W DornP V Halushka
Oct 1, 1987·Circulation·J H AshtonW B Campbell
Jan 2, 1985·European Journal of Pharmacology·J Y JeremyP Dandona
May 18, 1984·European Journal of Pharmacology·T M Lincoln, V Fisher-Simpson
Nov 1, 1981·The American Journal of Physiology·R Loutzenhiser, C van Breemen
Apr 1, 1995·Journal of Applied Physiology·F IchinoseW M Zapol
Jan 1, 1995·Journal of Applied Physiology·D M LindeborgR G Pearl
Apr 1, 1994·Shock·H NodaD L Traber
Feb 1, 1995·Journal of Applied Physiology·S A KraftR G Pearl
Dec 1, 1994·Circulation·S Fuse, T Kamiya
Citations
Jun 6, 2003·European Journal of Pharmacology·Wing Hung LauWui Hang Cheung
Apr 3, 2001·General Pharmacology·A van der ZyppH Majewski
Dec 12, 2001·British Journal of Pharmacology·L WalchX Norel
Apr 27, 2002·British Journal of Pharmacology·Javier AnguloIñigo Sáenz De Tejada
Aug 19, 2003·British Journal of Pharmacology·Federico Manuel DarayRodolfo Pedro Rothlin
Jul 17, 1999·The American Journal of Physiology·J BauerN Parekh