Pharmacological preconditioning by diazoxide downregulates cardiac L-type Ca(2+) channels.

British Journal of Pharmacology
G GonzálezJa Sánchez

Abstract

Pharmacological preconditioning (PPC) with mitochondrial ATP-sensitive K(+) (mitoK(ATP) ) channel openers such as diazoxide, leads to cardioprotection against ischaemia. However, effects on Ca(2+) homeostasis during PPC, particularly changes in Ca(2+) channel activity, are poorly understood. We investigated the effects of PPC on cardiac L-type Ca(2+) channels. PPC was induced in isolated hearts and enzymatically dissociated cardiomyocytes from adult rats by preincubation with diazoxide. We measured reactive oxygen species (ROS) production and Ca(2+) signals associated with action potentials using fluorescent probes, and L-type currents using a whole-cell patch-clamp technique. Levels of the α(1c) subunit of L-type channels in the cellular membrane were measured by Western blot. PPC was accompanied by a 50% reduction in α(1c) subunit levels, and by a reversible fall in L-type current amplitude and Ca(2+) transients. These effects were prevented by the ROS scavenger N-acetyl-L-cysteine (NAC), or by the mitoK(ATP) channel blocker 5-hydroxydecanoate (5-HD). PPC significantly reduced infarct size, an effect blocked by NAC and 5-HD. Nifedipine also conferred protection against infarction when applied during the reperfusion period. Do...Continue Reading

References

Sep 1, 1989·Journal of Molecular and Cellular Cardiology·M KanekoN S Dhalla
Jul 1, 1987·Cardiovascular Research·D Garcia-DoradoF Fernandez Aviles
Oct 1, 1994·Pflügers Archiv : European journal of physiology·J P GomezG Raymond
Oct 1, 1994·Cardiovascular Research·R S Vander HeideK A Reimer
Apr 12, 1996·The Journal of Biological Chemistry·K D GarlidP A Schindler
Aug 1, 1996·Journal of Molecular and Cellular Cardiology·D R WallbridgeG Heusch
Jan 1, 1997·Journal of Molecular and Cellular Cardiology·C P BainesJ M Downey
Nov 27, 1999·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·J Striessnig
Oct 14, 2000·Annual Review of Cell and Developmental Biology·W A Catterall
Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·C Pignier, D Potreau
Dec 21, 2000·American Journal of Physiology. Heart and Circulatory Physiology·S WangY Liu
Oct 16, 2001·The Journal of Physiology·J A SánchezS S Sheu
Jan 24, 2002·Nature·Donald M Bers
Jul 5, 2003·Physiological Reviews·Darrell E GollJinyang Cong
Sep 12, 2003·Journal of Molecular and Cellular Cardiology·Olaf OldenburgJames M Downey
Jun 28, 2005·Journal of Molecular and Cellular Cardiology·Hossein Ardehali, Brian O'Rourke
Dec 3, 2005·The Journal of Clinical Investigation·Ilona BodiArnold Schwartz
Mar 17, 2007·Archives of Biochemistry and Biophysics·Tobias JungTilman Grune
Jul 17, 2007·Biochimica Et Biophysica Acta·Andrew P HalestrapIgor Khaliulin
Sep 8, 2007·Progress in Biophysics and Molecular Biology·Ian FindlayYoshihisa Kurachi
Apr 9, 2008·Physiological Reviews·Elizabeth Murphy, Charles Steenbergen
Nov 4, 2009·British Journal of Pharmacology

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Citations

Jun 25, 2013·Pharmacology & Therapeutics·William A Coetzee
Oct 15, 2013·Canadian Journal of Physiology and Pharmacology·Rosario SolisJorge A Sánchez
Mar 10, 2018·International Journal of Molecular Sciences·Michela PecoraroAda Popolo
Oct 19, 2017·Journal of Cardiovascular Pharmacology·Jazmin Flores-MonroyLuisa Martínez-Aguilar
Apr 9, 2015·Journal of Cardiovascular Pharmacology·Francisco Rodrigo Lemos CaldasHeberty di Tarso Fernandes Facundo
Nov 8, 2018·Journal of Cardiovascular Pharmacology·Gayathri NarasimhanJorge A Sánchez
Nov 12, 2014·World Journal of Gastroenterology : WJG·Mateus Antunes NogueiraMarcel Cerqueira Cesar Machado

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