Pharmacological properties of the mouse neurotensin receptor 3. Maintenance of cell surface receptor during internalization of neurotensin

FEBS Letters
V NavarroJ Mazella

Abstract

We recently reported the molecular identification of a new type of receptor for the neuropeptide neurotensin (NT), the neurotensin receptor 3 (NTR3), identical to sortilin, which binds receptor-associated protein. Here, we demonstrate that the cloned mouse NTR3 is expressed on the plasma membrane of transfected COS-7 cells. The mouse NTR3 is detectable by photoaffinity labeling and immunoblotting at the cell surface as a 100 kDa N-glycosylated protein. Biochemical analysis and confocal microscopic imaging clearly indicate that NT is efficiently internalized after binding to NTR3, and that despite this internalization, the amount of receptor present on the cell surface is maintained.

References

Jan 31, 1997·The Journal of Biological Chemistry·G GaudriaultJ P Vincent
Sep 26, 1997·The Journal of Biological Chemistry·B Z LinK V Kandror
Mar 7, 1998·The Journal of Biological Chemistry·N J MorrisG E Lienhard
Oct 3, 1998·The Journal of Biological Chemistry·J MazellaJ P Vincent
Feb 2, 1999·The EMBO Journal·C Munck PetersenP Madsen
Mar 20, 1999·The Journal of Biological Chemistry·M S NielsenC M Petersen

❮ Previous
Next ❯

Citations

May 13, 2009·Cellular and Molecular Life Sciences : CMLS·Guido Hermey
Apr 12, 2016·Communicative & Integrative Biology·Cornelia M WilsonMarie-Odile Jauberteau
Jul 9, 2010·The Journal of Investigative Dermatology·Mária KissLajos Kemény
Mar 11, 2004·Journal of Neurochemistry·Guido HermeyIrm Hermans-Borgmeyer
Mar 5, 2008·Traffic·Morten S NielsenGuido Hermey
Nov 3, 2010·Alcoholism, Clinical and Experimental Research·Moonnoh R LeeDoo-Sup Choi
Oct 8, 2004·Brain Research. Molecular Brain Research·Pieter J PeetersThomas Steckler
Aug 18, 2004·The International Journal of Biochemistry & Cell Biology·Anne MorinvilleJean Mazella
Jul 29, 2006·Peptides·Jean Mazella, Jean-Pierre Vincent
Aug 12, 2006·Peptides·Jean Mazella, Jean-Pierre Vincent
Aug 8, 2006·Peptides·Ricardo CácedaCharles B Nemeroff
Mar 28, 2006·Biochemical and Biophysical Research Communications·Amélie PerronAlain Beaudet
Nov 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Arti B PatelTheoharis C Theoharides
Jul 18, 2014·Biology of Reproduction·Yuuki HiradateKentaro Tanemura
Oct 27, 2017·Cellular and Molecular Neurobiology·Ibrahim MalikGunnar P H Dietz
May 15, 2003·The Journal of Comparative Neurology·Philippe SarretAlain Beaudet
Jun 16, 2005·Journal of Neuroscience Research·Stéphane MartinJean Mazella
Dec 26, 2001·The Journal of Pharmacology and Experimental Therapeutics·Douglas J PettiboneZhizhen Zeng
Jun 22, 2019·Science Advances·Mette RichnerAnders Nykjær
May 18, 2006·The Journal of Clinical Endocrinology and Metabolism·Flavie SicardCatherine Delarue
Feb 23, 2011·Natural Product Reports·Charles R RobertsonGrzegorz Bulaj
Sep 15, 2004·The Journal of Biological Chemistry·Uffe B WestergaardClaus Munck Petersen
Jun 27, 2002·The Journal of Biological Chemistry·Philippe SarretAlain Beaudet
May 26, 2009·ACS Chemical Biology·Rebecca M MyersSteven V Ley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.