Pharmacologically active microcarriers for endothelial progenitor cell support and survival

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
Claudia MusilliAstrid Parenti

Abstract

The regenerative potential of endothelial progenitor cell (EPC)-based therapies is limited due to poor cell viability and minimal retention following application. Neovascularization can be improved by means of scaffolds supporting EPCs. The aim of the present study was to investigate whether human early EPCs (eEPCs) could be efficiently cultured on pharmacologically active microcarriers (PAMs), made with poly(d,l-lactic-coglycolic acid) and coated with adhesion/extracellular matrix molecules. They may serve as a support for stem cells and may be used as cell carriers providing a controlled delivery of active protein such as the angiogenic factor, vascular endothelial growth factor-A (VEGF-A). eEPC adhesion to fibronectin-coated PAMs (FN-PAMs) was assessed by means of microscopic evaluation and by means of Alamar blue assay. Phospho ERK(1/2) and PARP-1 expression was measured by means of Western blot to assess the survival effects of FN-PAMs releasing VEGF-A (FN-VEGF-PAMs). The Alamar blue assay or a modified Boyden chamber assay was employed to assess proliferative or migratory capacity, respectively. Our data indicate that eEPCs were able to adhere to empty FN-PAMs within a few hours. FN-VEGF-PAMs increased the ability of eEPC...Continue Reading

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Citations

Aug 30, 2013·Journal of Biomedical Science·Claudio MuscariCarlo Guarnieri
Jul 8, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jean-Pierre KaramN Claudia Montero-Menei
Aug 21, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·P Díaz-HerráezM J Blanco-Prieto
Jan 12, 2013·Journal of Cellular and Molecular Medicine·Claudia PennaPasquale Pagliaro
Aug 8, 2014·Journal of Biomedical Materials Research. Part a·Jean-Pierre KaramClaudia N Montero-Menei
Dec 21, 2014·Journal of Physiology and Biochemistry·Francesca BonafèClaudio Muscari
Mar 19, 2021·Critical Reviews in Biotechnology·Alonso Ornelas-GonzálezMarco Rito-Palomares

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