Pharmacologically and functionally distinct calcium currents of stomatogastric neurons

Journal of Neurophysiology
L M Hurley, K Graubard

Abstract

Previous studies have suggested the presence of different types of calcium channels in different regions of stomatogastric neurons. We sought to pharmacologically separate these calcium channel types. We used two different preparations from different regions of stomatogastric neurons to screen a range of selective calcium channel blockers. The two preparations were isolated cell bodies in culture, in which calcium current was measured directly, and isolated neuromuscular junction, in which synaptic transmission was the indirect assay for presynaptic calcium influx. The selective blockers were two different dihydropyridines, omega-Agatoxin IVA, and omega-Conotoxin GVIA. Cultured cell bodies possessed both high-threshold calcium current and calcium-activated outward current, similar to intact neurons. The calcium current had transient and maintained components, but both components had the same voltage dependence of activation and inactivation. Dihydropyridines at >/=10 microM blocked both high-threshold calcium current and calcium-activated outward current. Nanomolar doses of omega-Agatoxin IVA did not block calcium current, but micromolar doses did. omega-Conotoxin GVIA did not block either current. In contrast, at the neuromusc...Continue Reading

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Citations

Aug 9, 2003·Journal of Neurophysiology·Bruce R JohnsonRonald M Harris-Warrick
Jul 29, 2005·Journal of Neurophysiology·Jason N MacLeanRonald M Harris-Warrick
Nov 18, 2005·Journal of Neurophysiology·Thuc LeJohn T Birmingham
Jun 30, 2006·Journal of Neurophysiology·Rodolfo J Haedo, Jorge Golowasch

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