Phase-changing sacrificial materials for interfacing microfluidics with ion-permeable membranes to create on-chip preconcentrators and electric field gradient focusing microchips

Analytical Chemistry
Ryan T KellyAdam T Woolley

Abstract

We have developed a novel approach for interfacing ionically conductive membranes with microfluidic systems using phase-changing sacrificial layers. Imprinted microchannels in a polymer substrate are filled with a heated liquid that solidifies at room temperature, a monomer solution is placed over the protected channels and polymerized to form a rigid semipermeable copolymer, and then the protective layer is melted and removed, leaving an open microchannel interfaced with a polymer membrane. We have applied this method in miniaturizing electric field gradient focusing (EFGF) and carrying out on-chip protein preconcentration. A semipermeable copolymer in the EFGF microchips fills a region of changing cross-sectional area, which allows a gradient in electric field to be established when an electrical potential is applied. Our technique provides microchip EFGF devices that offer 3-fold improved resolution in protein focusing compared with capillary-based systems. In addition, these EFGF microchips can separate peptide samples with resolution similar to what is obtained in capillary electrophoresis microdevices, and the micro-EFGF systems enrich analytes by a factor of >150. Finally, we have fabricated membrane-integrated microflui...Continue Reading

References

Jun 13, 1998·Biotechnology Progress·R D Greenlee, C F Ivory
Jul 25, 2000·Analytical Chemistry·J KhandurinaJ M Ramsey
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Brian M PaegelRichard A Mathies
Feb 13, 2003·Journal of Chromatography. a·Qinggang WangMilton L Lee
Apr 26, 2003·Analytical Chemistry·Ryan T Kelly, Adam T Woolley
Jun 15, 2004·Analytical Chemistry·Torsten VilknerAndreas Manz
Sep 10, 2004·Journal of Chromatography. a·P Myers, K D Bartle
Dec 30, 2004·Analytical Chemistry·Robert S FooteJ Michael Ramsey
Nov 10, 2005·Journal of Separation Science·Ryan T Kelly, Adam T Woolley

❮ Previous
Next ❯

Citations

Nov 15, 2006·Analytical and Bioanalytical Chemistry·Jonathan G ShackmanDavid Ross
Oct 7, 2008·Analytical and Bioanalytical Chemistry·Daniel J Eves, Adam T Woolley
Jan 11, 2011·Analytical Chemistry·Brian E RootAnnelise E Barron
Nov 23, 2007·Analytical Chemistry·Hernan V Fuentes, Adam T Woolley
Oct 2, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Sachio Yamamoto
Mar 7, 2018·Genes·Samar DamiatiEva-Kathrin Ehmoser
Dec 9, 2014·Lab on a Chip·Thierry Leïchlé, David Bourrier
Aug 25, 2006·Lab on a Chip·J de JongM Wessling
Nov 23, 2006·Electrophoresis·Bridget A PeeniAdam T Woolley
Jan 25, 2007·Electrophoresis·Cornelius F Ivory
Jul 12, 2007·Journal of Separation Science·Alexandra Höltzel, Ulrich Tallarek
Dec 7, 2007·Electrophoresis·Youyuan PengMyriam Taverna
May 24, 2008·Lab on a Chip·Hao LinDavid Ross
Nov 28, 2008·Electrophoresis·Ken-ichi OhnoAndreas Manz
Jun 18, 2009·Lab on a Chip·Dzmitry HlushkouUlrich Tallarek
Mar 2, 2010·Electrophoresis·Jeffrey M Burke, Cornelius F Ivory
Aug 12, 2008·Journal of Separation Science·Kenji SueyoshiKoji Otsuka
Feb 17, 2007·Electrophoresis·Jonathan G Shackman, David Ross
Aug 15, 2008·Electrophoresis·Weichun YangAdam T Woolley
May 24, 2008·Analytical Chemistry·Jonathan WestAndreas Manz
Nov 20, 2009·Analytical Chemistry·Robbyn K PerdueRichard M Crooks

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.