Phase-dependent redox insulation in mussel adhesion

Science Advances
Eric ValoisJ Herbert Waite

Abstract

Catecholic 3,4-dihydroxyphenyl-l-alanine (Dopa) residues in mussel foot proteins (mfps) contribute critically to mussel (Mytilus californianus) plaque adhesion, but only if protected from oxidation at the adhesive-substratum interface. Dopa oxidation is thermodynamically favorable in seawater yet barely detectable in plaques; therefore, we investigated how plaques insulate Dopa-containing mfps against oxidation. Seawater sulfate triggers an mfp3 and mfp6 liquid-liquid phase separation (LLPS). By combining plaque cyclic voltammetry with electrophoresis, mass spectrometry, and redox-exchange chemistry, we show that Dopa-containing mfp3 and mfp6 in phase-separated droplets remain stable despite rapid oxidation in the surrounding equilibrium solution. The results suggest that a cohort of oxidation-prone proteins is endowed with phase-dependent redox stability. Moreover, in forming LLPS compartments, Dopa proteins become reservoirs of chemical energy.

References

Mar 1, 1969·Archives of Biochemistry and Biophysics·S Panyim, R Chalkley
Aug 29, 2003·Nature·Hyoung-Joon Jin, David L Kaplan
Oct 18, 2005·The Journal of Biological Chemistry·Hua ZhaoJ Herbert Waite
Feb 24, 2006·The Journal of Biological Chemistry·Hua ZhaoJ Herbert Waite
Jul 18, 2006·The Journal of Biological Chemistry·Hua Zhao, J Herbert Waite
May 6, 2011·Soft Matter·Dong Soo HwangJ Herbert Waite
Aug 2, 2011·Nature Chemical Biology·Jing YuJ Herbert Waite
Nov 28, 2012·Journal of the American Chemical Society·Jinhwa HeoCraig J Hawker
Dec 12, 2012·Journal of the American Chemical Society·Wei WeiJ Herbert Waite
Jul 9, 2013·Zoological Science·Masahiro YamanakaYoshihiro Fukumori
Sep 26, 2013·Acta Biomaterialia·Wei WeiJ Herbert Waite
Sep 23, 2014·Nature Nanotechnology·Chao ZhongTimothy K Lu
Oct 8, 2014·Annual Review of Cell and Developmental Biology·Anthony A HymanFrank Jülicher
Apr 16, 2015·Biofouling·Nadine R Martinez RodriguezJ Herbert Waite
Jun 9, 2015·Nature Chemical Biology·YerPeng TanJ Herbert Waite
Oct 16, 2015·Journal of the Royal Society, Interface·Dusty R MillerJ Herbert Waite
Dec 4, 2015·Journal of the Royal Society, Interface·Emmanouela FilippidiMegan T Valentine
Jan 9, 2016·Biochemistry·Razieh MirshafianJohn Herbert Waite
Feb 17, 2017·The Journal of Experimental Biology·J Herbert Waite
Jun 1, 2017·Nature·Ankur Jain, Ronald D Vale
Jun 9, 2017·Journal of the Royal Society, Interface·Daniel G DeMartiniJ Herbert Waite
Sep 25, 2017·Science·Yongdae Shin, Clifford P Brangwynne
Dec 22, 2017·Biomaterials Science·Greg P MaierAlison Butler
Mar 25, 2018·Angewandte Chemie·J Saiz-PoseuD Ruiz-Molina
Aug 4, 2018·Biomacromolecules·Jia Wang, Thomas Scheibel
Aug 26, 2018·Nature Communications·Christophe A MonnierJ Herbert Waite

❮ Previous
Next ❯

Citations

Sep 17, 2020·Soft Matter·Justin H BernsteinMegan T Valentine
Feb 18, 2021·Biological Reviews of the Cambridge Philosophical Society·Peter A DaveyNick Aldred
Jan 13, 2021·Angewandte Chemie·Ayala LampelRein V Ulijn
Jun 10, 2021·The Journal of Physical Chemistry. B·Jessica LimKonstantin Pervushin
Aug 3, 2021·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Esther Amstad, Matthew J Harrington
Oct 9, 2021·Chemistry : a European Journal·Jing SunHongjie Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
scanning electron microscopy
x-ray photoelectron spectroscopy
electrophoresis
electron microscopy
electron tomography

Software Mentioned

Casa

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.