Phase diagram of lipid A from Salmonella minnesota and Escherichia coli rough mutant lipopolysaccharide

Journal of Structural Biology
Klaus BrandenburgU Seydel

Abstract

We have reported here on the structural polymorphism of lipid A, the "endotoxic principle" of bacterial lipopolysaccharide. For lipid A of rough mutant lipopolysaccharide from Salmonella minnesota and Escherichia coli, the three-dimensional supramolecular structures were determined with x-ray diffraction utilizing synchrotron radiation. The investigations were performed in the water concentration range 10 to 95% by weight, at [lipid A]:[Mg2+] molar ratios from 1:0 to 0.1:1, and in the temperature range from 20 to 70 degrees C. These data were correlated with measurements of the beta----alpha phase behaviour which was monitored with differential scanning calorimetry and Fourier-transform infrared spectroscopy. We found that the transition temperature of the acyl chains ranges--in the absence of Mg2(+)-from 45 degrees C at high to 56 degrees C at low water content, and-at an equimolar content of Mg2(+)-from 52 degrees C at high to 59 degrees C at low water concentrations. In the gel phase-in which the lipid A acyl chains are more disordered than those from saturated phospholipids-cubic phases are adopted at high water content (greater than 60%) and at high [lipid A]:[Mg2+] molar ratios. At low water contents, lamellar states are ...Continue Reading

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Citations

Dec 13, 2003·Carbohydrate Research·Klaus BrandenburgPatrick Garidel
Jul 8, 2010·Antimicrobial Agents and Chemotherapy·Thomas GutsmannKlaus Brandenburg
Jan 24, 2016·Biochimica Et Biophysica Acta·Klaus BrandenburgKarl Lohner
Oct 26, 1999·Drug Development and Industrial Pharmacy·Y Asai, S Watanabe
Jun 7, 2011·Biophysical Journal·Yani KaconisKlaus Brandenburg
Dec 10, 2015·Scientific Reports·Teresa ParamoPeter J Bond
Jan 1, 1992·Journal of Structural Biology·S Gerbl-RiegerW Baumeister
Dec 1, 1993·European Journal of Biochemistry·K BrandenburgU Seydel
Nov 2, 2017·Science Signaling·Carmen L KrügerMike Heilemann
Aug 9, 2020·Frontiers in Cell and Developmental Biology·Laura PaulowskiThomas Gutsmann
Nov 15, 2013·Chemical Communications : Chem Comm·Agrinaldo NascimentoThereza A Soares
Jul 7, 2018·International Journal of Molecular Sciences·Maros KolomaznikAndrea Calkovska
Feb 28, 2020·Biochimica Et Biophysica Acta. General Subjects·Antonio De NicolaGiuseppe Milano
Oct 11, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Götz MilkereitRegine Willumeit
Sep 1, 1996·Progress in Lipid Research·S G Wilkinson
Jun 26, 2021·Pharmacology & Therapeutics·Cong LinXiaohui Wang
Mar 1, 1992·Journal of Structural Biology·K BrandenburgU Seydel
Jul 14, 2021·Physical Chemistry Chemical Physics : PCCP·Andresa MessiasThereza A Soares
Oct 24, 2012·The Journal of Physical Chemistry. B·Chester A Faunce, Henrich H Paradies
Jul 11, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·James JenningsMahesh K Mahanthappa

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