Phase II enzyme inducer, sulforaphane, inhibits UVB-induced AP-1 activation in human keratinocytes by a novel mechanism.

Molecular Carcinogenesis
Ming ZhuG Timothy Bowden

Abstract

Ultraviolet (UV) light-induced activation of activator protein-1 (AP-1), resulting at least in part from oxidative stress, promotes skin carcinogenesis. It has not yet been determined whether elevating cellular phase II enzymes and glutathione (GSH) levels inhibits the AP-1 activation. We have, therefore, examined the effects of two well-known inducers of phase II enzymes, sulforaphane (SF) and tert-butylhydroquinone (tBHQ), on UVB-induced AP-1 activation, with an AP-1-luciferase reporter plasmid that was stably transfected into human HaCaT keratinocytes (HCL14 cells). Exposure of HCL14 cells to SF or tBHQ led to the induction of quinone reductase-1 (QR-1), a marker of global cellular phase II enzymes, as well as elevation of cellular GSH levels. Incubation of the cells with 1-10 microM SF or 11-45 microM tBHQ for 24 h resulted in up to 1.4-fold and 1.7-fold increase of QR-1 activity, respectively, and up to 1.5-fold and 1.6-fold increases in cellular GSH levels, respectively. AP-1 activation was dramatically enhanced by irradiating HCL14 cells with 250 J/m(2) of UVB. While the above SF treatment dose-dependently reduced the UVB-induced AP-1 activation in HCL14 cells, the tBHQ treatment did not, suggesting that elevating cellul...Continue Reading

References

Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·Y ZhangG H Posner
Dec 1, 1990·Photochemistry and Photobiology·H N Ananthaswamy, W E Pierceall
Oct 1, 1986·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R Rehwoldt
Jan 1, 1997·Journal of Photochemistry and Photobiology. B, Biology·M Garmyn, H Degreef
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·C HuangZ Dong
Jul 29, 1998·Chemico-biological Interactions·M E Anderson
May 8, 1999·The Journal of Investigative Dermatology·D PeusM R Pittelkow
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·M R YoungN Colburn
Oct 29, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J W Fahey, P Talalay
Nov 5, 1999·Carcinogenesis·K Kramer-SticklandG T Bowden
Jun 19, 2001·The Journal of Biological Chemistry·E HeissC Gerhäuser
Jan 30, 2002·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael Thun
May 4, 2002·Nature Cell Biology·Eitan Shaulian, Michael Karin
Aug 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Albena T Dinkova-KostovaPaul Talalay
Nov 22, 2002·Journal of Dermatological Science·Yuesheng ZhangMin Jian Xu
May 6, 2003·The Journal of Biological Chemistry·Diane E HeckJeffrey D Laskin

❮ Previous
Next ❯

Citations

Mar 12, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Xi-Lin ChenCharles Kunsch
Dec 15, 2010·Archives of Biochemistry and Biophysics·Farrukh Afaq
Aug 2, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Ming-Jenn ChenYi-Chiang Hsu
Jan 23, 2016·Journal of Cosmetic Dermatology·Sohely SikdarJacques Dubois
Dec 20, 2011·Mutation Research·Carmela FimognariPatrizia Hrelia
Apr 21, 2009·Biochemical Pharmacology·Xiu-Hong WangGraham Packham
Jan 27, 2009·Clinics in Dermatology·Anika E Wagner, Gerald Rimbach
Nov 7, 2012·Photodermatology, Photoimmunology & Photomedicine·Ki Cheon KimJin Won Hyun
Mar 8, 2006·Cancer Letters·Melinda C Myzak, Roderick H Dashwood
Jan 8, 2017·Photochemistry and Photobiology·Mary K Montes de OcaFarrukh Afaq
Aug 29, 2007·Acta Pharmacologica Sinica·Ki Han KwonAh-Ng Tony Kong
Jan 30, 2007·The Journal of Pharmacology and Experimental Therapeutics·Ji Man HanOnyou Hwang
Dec 1, 2009·Toxicological Research·Jiyoung Kim, Young-Joon Surh
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Junxiang Wan, David Diaz-Sanchez
Apr 4, 2019·GeroScience·Roberto Santín-MárquezMina Königsberg
Jul 6, 2020·Current Research in Translational Medicine·Swati ChadhaSandeep Arora

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.