PMID: 8953221Oct 1, 1996Paper

Phase labeling of C-H and C-C spin-system topologies: application in constant-time PFG-CBCA(CO)NH experiments for discriminating amino acid spin-system types

Journal of Biomolecular NMR
C B RiosG T Montelione

Abstract

Triple-resonance experiments facilitate the determination of sequence-specific resonance assignments of medium-sized 13C,15N-enriched proteins. Some triple-resonance experiments can also be used to obtain information about amino acid spin-system topologies by proper delay tuning. The constant-time PFG-CBCA(CO)NH experiment allows discrimination between five different groups of amino acids by tuning (phase labeling) independently the delays for proton-carbon refocusing and carbon-carbon constant-time frequency labeling. The proton-carbon refocusing delay allows discrimination of spin-system topologies based on the number of protons attached to C alpha and C beta atoms (i.e. C-H phase labeling). In addition, tuning of the carbon-carbon constant-time frequency-labeling delay discriminates topologies based on the number of carbons directly coupled to C alpha and C beta atoms (i.e. C-C phase labeling). Classifying the spin systems into these five groups facilitates identification of amino acid types, making both manual and automated analysis of assignments easier. The use of this pair of optimally tuned PFG-CBCA(CO)NH experiments for distinguishing five spin-system topologies is demonstrated for the 124-residue bovine pancreatic rib...Continue Reading

References

Jan 1, 1996·Journal of Magnetic Resonance. Series B·V DötschG Wagner
Oct 1, 1995·Current Opinion in Structural Biology·D E Zimmerman, G T Montelione
Jun 1, 1996·Journal of Magnetic Resonance. Series B·V Dötsch, G Wagner
Jan 1, 1996·Journal of Biomolecular NMR·B T Farmer, R A Venters

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Citations

Sep 5, 2012·Journal of Biomolecular NMR·David Pantoja-Uceda, Jorge Santoro
Jul 21, 2011·Veterinary Medicine International·Claire BarrySam Strain
Feb 20, 2016·Journal of Biomolecular NMR·Alessandro PiaiAnna Zawadzka-Kazimierczuk
Oct 3, 2008·Journal of Magnetic Resonance·David Pantoja-Uceda, Jorge Santoro
Jan 3, 2001·Journal of Magnetic Resonance·M SchubertP Schmieder
Oct 6, 1999·Current Opinion in Structural Biology·H N Moseley, G T Montelione
Jul 31, 2013·Journal of Magnetic Resonance·David Pantoja-Uceda, Jorge Santoro
Oct 12, 2004·Biophysical Journal·Norma J GreenfieldSarah E Hitchcock-DeGregori
Mar 25, 1999·Journal of Magnetic Resonance·G V Swapna, G T Montelione
Oct 21, 1999·Journal of Magnetic Resonance·M SchubertH Oschkinat
Mar 19, 2008·Journal of the American Chemical Society·Ewen LescopBernhard Brutscher

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