Phase separation of Arabidopsis EMB1579 controls transcription, mRNA splicing, and development

PLoS Biology
Yiling ZhangShanjin Huang

Abstract

Tight regulation of gene transcription and mRNA splicing is essential for plant growth and development. Here we demonstrate that a plant-specific protein, EMBRYO DEFECTIVE 1579 (EMB1579), controls multiple growth and developmental processes in Arabidopsis. We demonstrate that EMB1579 forms liquid-like condensates both in vitro and in vivo, and the formation of normal-sized EMB1579 condensates is crucial for its cellular functions. We found that some chromosomal and RNA-related proteins interact with EMB1579 compartments, and loss of function of EMB1579 affects global gene transcription and mRNA splicing. Using floral transition as a physiological process, we demonstrate that EMB1579 is involved in FLOWERING LOCUS C (FLC)-mediated repression of flowering. Interestingly, we found that EMB1579 physically interacts with a homologue of Drosophila nucleosome remodeling factor 55-kDa (p55) called MULTIPLE SUPPRESSOR OF IRA 4 (MSI4), which has been implicated in repressing the expression of FLC by forming a complex with DNA Damage Binding Protein 1 (DDB1) and Cullin 4 (CUL4). This complex, named CUL4-DDB1MSI4, physically associates with a CURLY LEAF (CLF)-containing Polycomb Repressive Complex 2 (CLF-PRC2). We further demonstrate that ...Continue Reading

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Citations

Apr 30, 2021·EMBO Reports·Jiwoo KimPil Joon Seo
Jul 28, 2021·Journal of Integrative Plant Biology·Xiumei XuLixin Zhang
Sep 30, 2021·The Plant Journal : for Cell and Molecular Biology·Zhen LeiJungnam Cho

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Datasets Mentioned

BETA
EYU30242

Methods Mentioned

BETA
reverse transcription PCR
fluorescence recovery after photobleaching
pull-down
RNA-seq
transgenic
confocal microscopy
two-hybrid
ChIP-seq
immunoprecipitation
dissection

Software Mentioned

ImageJ
ExPASy
TopHat
MEGA5

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