Phase separation of cholesterol and the interaction of ethanol with phosphatidylserine-cholesterol bilayer membranes

Chemistry and Physics of Lipids
D BachE J Wachtel

Abstract

Thermotropic and structural effects of ethanol on phosphatidylserine (PS) membranes containing up to 0.4 mol fraction cholesterol were investigated by differential scanning calorimetry, X-ray diffraction and fluorescence spectroscopy. It was found that in the presence of cholesterol, 10% (v/v) added ethanol depresses the melting temperature of the phospholipid by approximately 2 degrees C, similar to what was observed in the absence of cholesterol. Below the melting temperature the progressive disordering effect of added cholesterol is weakly enhanced by the presence of ethanol. In the liquid crystalline state, the marked decrease in the thickness of the bilayer which ethanol causes in the absence of cholesterol (Chem. Phys. Lipids 92 (1998) 127), is also observed in its presence. We conclude that, in contrast to what has been observed for zwitterionic phospholipids, high concentrations of cholesterol do not diminish the interaction of ethanol with PS membranes. With addition of 10% (v/v) ethanol, crystalline cholesterol diffraction, an indication of phase separation of the sterol, appears at mol fraction cholesterol 0.34, as compared to 0.3 in the absence of ethanol (Chem. Phys. Lipids 92 (1998) 71).

References

Jan 1, 1988·Progress in Lipid Research·J L Slater, C H Huang
Nov 21, 1987·Biochimica Et Biophysica Acta·E J Wachtel, D Bach
Apr 9, 1987·Biochimica Et Biophysica Acta·M BentalS Nir
Jul 1, 1981·Biochemical Pharmacology·D Bach, B Sela
Apr 7, 1998·Biochimica Et Biophysica Acta·O P BondarE S Rowe
Jul 31, 1998·Chemistry and Physics of Lipids·E WachtelI R Miller

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Citations

Apr 28, 2005·Chemistry and Physics of Lipids·Richard M EpandEllen Wachtel
Jan 5, 2006·Journal of the American Chemical Society·Jianbing ZhangSteven L Regen
Oct 17, 2003·Biochimica Et Biophysica Acta·Richard M EpandEllen Wachtel
Mar 22, 2003·Biochimica Et Biophysica Acta·Diana Bach, Ellen Wachtel
Jan 9, 2003·Journal of Lipid Research·Sofiya KolushevaRaz Jelinek

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